Abstract
Nitric oxide (NO) and Jasmonic acid (JA) are two key signaling molecules involved in many and diverse biological pathways in plants. Growing evidence suggested that NO signaling interacts with JA signaling. In this work, Our experiment showed that NO exists in guard cell ofVicia faba L., and NO is involved in signal transduction of JA-induced stomata closuring: (i) JA enhances NO synthesis in guard cell; (ii) both JA and NO induced stomatal closure, and had dose response to their effects; (iii) there are synergetic correlation between JA and lower NO concentration in regulation of stomatal movement; (iv) JA-induced stomatal closure was largely prevented by 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), a specific NO scavenger. An inhibitor of NO synthase (NOS) in mammalian cells,N G-nitro-L-Arg-methyl eater (L-NAME) also inhibits plant NOS, repressing JA-induced NO generation and JA-induced stomatal closure. We presumed that NO mainly comes from NOS after JA treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dat, J., Vandenbeele, S., Vranova, E. et al., Dual action of the active oxygen species during plant stress responses, Cellular Mol. Life Sci., 2000, 57: 779–795.
Delledonne, M., Xia, Y. J., Dixon, R. A. et al., Nitric oxide functions as a signal in plant disease resistance, Nature, 1998, 394: 585–588.
Garcia-Mata, C., Lamattina, L., Abscisic acid, nitric oxide and stomatal closure: Is nitrate reductase one of the missing links? Trends Plant Sci., 2003, 8: 20–26.
Neill, S. J., Desikan, R., Hancock, J. T., Nitric oxide signaling in plants, New Phytologist, 2004, 159: 11–35.
Wendehenne, D., Pugin, A., Klessig, D. F., et al., Nitric oxide: Comparative synthesis and signaling in animal and plant cells, Trends Plant Sci., 2001, 6: 177–183.
Neill, S. J., Desikan, R., Clarke, A. et al., Nitric oxide is a novel component of abscisic acid signaling in stomatal guard cells, Plant Physiol., 2002, 128 (1): 13–16.
Gehring, C. A., Irving, H. R., McConchie, R. et al., Jasmonates induce intracellular alkalinizaton and closure ofPaphiopedilum guard cells, Ann. Bot, 1997, 80: 485–489.
Clarke, A., Desikan, R., Hurst, R. D. et al., NO way back: Nitric oxide and programmed cell death inArabidopsis thaliana suspension cultures, Plant J., 2000, 24(5): 667–677.
Seo, H. S., Song, J. T., Cheong, J. J. et al., Jasmonic acid carboxyl methyltransferase: A key enzyme for jasmonate-regulated plant responses, Proc. Natl. Acad. Sci. USA, 2001, 98(8): 4788–4793.
Xin, Z. Y., Zhou, X., Pilel, P. E., Level changes of jasmonic, abscisic, and indole-3yl-aceric acids in maize under desiccation stress, J. Plant Physiol., 1997, 151: 120–124.
Kramell, R., Miersch, O., Atzorn, R. et al., Octadecanoid-derived alteration of gene expression and the “Oxylipin signature” in stressed barley leaves, Plant Physiol., 2000, 123(1): 177–187.
Koch, J. R., Creelman, R. A., Eshita, S. M. et al., Ozone sensitivity in hybrid poplar correlates with insensitivity to both salicylic acid and jasmonic acid, Plant Physiol., 2000, 123: 1–10.
Huang, X., Stettmaier, K., Michel, C. et al., Nitric oxide is induced by wounding and influences jasmonic acid signaling inArabidopsis thaliana, Planta, 2004, 218: 938–946.
Foissner, I., Wendehenne, D., Langebartels, C. et al.,In vivo imaging of an elicitor-induced nitric oxide burst in tobacco, Plant J., 2000, 23: 817–824.
Cui, X. H., Miao, Y.C., Dong, F. C. et al., The initial research of mechanism of signal transduction of Methyl-Jasmonate-induced stomatal closure, J. Henan University (Natural Science), 2001, 31(1): 76–79.
Del Rio, L. A., Corpas, F. J., Barroso, J. B., Nitric oxide and nitric oxide synthase activity in plants, Phytochemistry, 2004, 65: 783–792.
Durner, J., Wendehenne, D., Klessig, D. F., Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose, Proc. Natl. Acad. Sci. USA. 1998, 95(17): 10328–10333.
Wojtaszek, P., Nitric oxide in plants: To NO or not to NO, Phytochem., 2000, 54(1): 1–4.
Desikan, R., Griths, R., Hancock, J. et al., A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure inArabidopsis thaliana, Proc. Natl. Acad. Sci. USA, 2002, 99: 16314–16318.
Rockel, P., Strube, F., Rockel, A. et al., Regulation of nitric oxide (NO) production by plant nitrate-reductasein vivo andin vitro, J. Exp. Bot, 2002, 53: 103–110.
Chandok, M. R., Ytterberg, A. J., van Wijk, K. L. et al., The pathogen-inducible nitric oxide synthase (iNOS) in plants is a variant of the P protein of the glycine decarboxylase complex, 2003, Cell, 113: 469–482.
Pedroso, M. C., Magalhaes, J. R. Durzan, D., Nitric oxide induces cell death in Taxus cells, Plant Sci., 2000, 157({Sn2}): 173–180.
Garced, H., Durzan, D., Pedroso, M. C., Mechanical stress elicits nitric oxide formation and DNA fragmentation inArabidopsis thaliana, Ann. Bot, 2001, 87({Sn5}): 567–574.
Orozco-Cardenas, M. L., Ryan, C. A., Nitric oxide negatively modulates wounding signaling in tomato plants, Plant Physiol., 2002, 130: 487–493.
Wendehenne, D., Durner, J., Klessig, D. F., Nitric oxide: A new player in plant signaling and defense responses, Curr. Opin. Plant Biol., 2004, 7: 449–455.
Liu, X., Zhang, S. Q., Lou, C. H., Involvement of nitric oxide in the signal transduction of salicylic acid regulating stomatal movement, Chinese Science Bulletin, 2003, 48(5): 449–452.
Guo, F. Q., Okamoto, M., Crawford, N. M., Identification of a plant nitric oxide synthase gene involved hormonal signaling, Science, 2003, 302: 100–103.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Liu, X., Shi, W., Zhang, S. et al. Nitric oxide involved in signal transduction of Jasmonic acid-induced stomatal closure ofVicia faba L.. Chin.Sci.Bull. 50, 520–525 (2005). https://doi.org/10.1007/BF02897475
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02897475