Abstract
A 6.0 kb DNA fragment related to nikkomycin biosynthesis was cloned from nikkomycin-producingStreptomyces ansochromogenes 7100. Sequence analysis showed that the 1.9 kbTth111 I fragment, a part of the 6.0 kb DNA fragment, contains one complete ORF designatedsanB (GenBank accession No. AF224501), which is composed of 1740 bp encoding a protein consisting of 580 amino acid residues. Its start codon is GTG at 100 bp position and stop codon is TGA at 1840-bp position. Database searching indicated that the deduced protein ofsanB is homologous to the histidinol-phosphate aminotransferase inStreptomyces coelicolor with 31% identities and 47% positives. Gene disruption was performed to study the function ofsanB. It was found that disruptants ofsanB lost the ability to synthesize nikkomycin, which reveals thatsanB is a novel gene essential for nikkomycin biosynthesis.
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Mueller, H., Funher, R., Zaehner, H. et al., Effect of nikkomycin Z, nikkomycin X and polyoxin A on chitosomalchitin synthetase, Arch Microbiol., 1981, 130: 195.
Fiedler, H. P., Kurth, R., Langhaerig, J. et al., Nikkomycins: microbial inhibitors of chitin synthetase, J. Chem. Biotechnol., 1982, 32:271.
Hector, R. F., Zimmer, B. L., Pappagianis, D., Evaluation of nikkomycins X and Z in murine models of coccidioidomycosis, histoplasmosis, and blastomycosis, Antimicrob Agents Chemother, 1990, 34: 587.
Bormann, C., Mattern, S., Fiedler, H. P. et al., Isolation ofStreptomyces tendae mutants with an altered nikkomycin spectrum, J. Antibiot., 1989, 42: 913.
Brunter, C., Lauer, B., Schwarz, W. et al., Molecular characterization of co-transcribed genes fromStreptomycestendae Tü901 involved in the biosynthesis of the peptidyl moiety of the peptidyl nucleoside antibiotic nikkomycin, Mol. Gen. Genet., 1999, 262: 102.
Schuez, T. C., Fiedler, H. P., Zaehner, H., Metabolic products Sz, Sx, Soz and Sox, new intermediates associated to the nikkomycin biosynthesis ofStreptomyces tendae, J. Antibiot., 1992, 45: 199.
Jia, J. Y., Li, W. L., Chen, W. et al., Cloning, sequencing and function ofsanA, a gene involved in nikkomycin biosynthesis ofStreptomyces ansochmmogenes, Science in China, Ser. C., 2000, 43(1): 30.
Manniatis, T., Frirsch, E. F., Sambrook, J., Molecular Cloning, A Laboratory Manual, New York: Cold Spring Harbor Laboratory Press, 1982.
Hopwood, D. A., Bibb, M. J., Chater, K. E. et al., Genetic Manipulation of Streptomyces, A Laboratory Manual, Norwich: John Innes Foundation, 1988.
Tan, H. R., Chater, K. F., Two developmentally controlled promoters ofStreptomyces coelicolor A3 (2) that resemble the major class of motility-related promoters in other bacteria. J. Bacteriol., 1993, 175(4): 933.
Bruntner, C., Bormann, C., TheStreptomyces tendae Tü901 L-lysine 2-aminotransferase catalyzes the initial reaction in nikkomycin D biosynthesis, Eur. J. Biochem., 1998, 254: 347.
Sanger, F., Nicklen, S., Coulson, A. R., DNA sequencing with Chain-terminating inhibitors, Proc. Natl. Acad. Sci. USA, 1997, 74: 5463.
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Li, W., Jia, J., Tan, H. et al. Cloning, sequencing and function ofsanB, a gene related to nikkomycin biosynthesis ofStreptomyces ansochromogenes . Chin. Sci. Bull. 45, 2158–2162 (2000). https://doi.org/10.1007/BF02886321
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DOI: https://doi.org/10.1007/BF02886321