Abstract
Two mutants of the hymenomyceteSchizophyllum commune, unable to use acetate as the sole carbon source for growth, were isolated. Growth of the mutants on a glucose minimal medium was only slightly inhibited by sodium azide. Genetic analysis revealed mutations in different chromosomal genes in the respective mutants. Both these mutants exhibited a high cyanide-insensitive endogenous respiration. The inhibition of the respiration by 8-hydroxyquinoline showed the mutants to respire predominantly by an alternative respiratory pathway observed in many fungal species, but not in the hymenomycetes so far. An enhanced cyanide-insensitive respiration was also found in a wild-type strain ofSchizophyllum commune grown in the presence of sodium azide.
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Bendall D. S., Bonner W. D. Jr.: Cyanide-insensitive respiration in plant mitochondria.Plant Physiol.47, 236 (1971).
Bonitati J., Miles P. G., Elliott W. B.: New technique of sample preparation for respirometry of filamentous fungi.Plant. Physiol.45, 708 (1970).
Casselton L. A., Casselton P. J.: Functional aspects of fluoroacetate resistance inCoprinus with special reference to acetyl-CoA synthetase deficiency.Mol. Gen. Genetics132, 255 (1974).
Casselton L. A., Condit A.: A mitochondrial mutant ofCoprinus lagopus.J. Gen. Microbiol.72, 521 (1972).
Clark-Walker G. D.: Development of respiration and mitochondria inMucor genevensis after anaerobic growth: absence of glucose repression.J. Bacteriol.109, 399 (1972).
Downie J. A., Garland P. B.: An antimycin A- and cyanide-resistant variant ofCandida utilis arising during copper-limited growth.Biochem. J.134, 1051 (1973).
Edwards D. L., Kwiecinski F.: Altered mitochondrial respiration in a chromosomal mutant ofNeurospora crassa.J. Bacteriol.116, 610 (1973).
Edwards D. L., Rosenberg E., Maroney P. A.: Induction of cyanide-insensitive respiration inNeurospora crassa.J. Biol. Chem.249, 3551 (1974).
Evans D. A., Brown R. C.: m-Chlorobenzhydroxamic acid-an inhibitor of cyanide-insensitive respiration inTrypanosoma brucei.J. Protozool.20, 157 (1973).
Flavell R. B., Fincham J. R. S.: Acetate-nonutilizing mutants ofNeurospora crassa. I. Mutant isolation, complementation studies and linkage relationships.J. Bacteriol.95, 1056 (1968).
Fry W. E., Millar R. L.: Cyanide tolerance inStemphylium loti.Phytopathology61, 494 (1971).
Grimm P. W., Allen P. J.: Promotion by zinc of the formation of cytoohromes inUstilago sphaerogena.Plant. Physiol.29, 369 (1954).
Hanssens L., D’Hondt E., Verachtert H.: Cyanide-insensitive respiration inMoniliella tomentosa and the effect of drugs on respiration and polyol biosynthesis.Arch. Microbiol.98, 339 (1974).
Hoffman R. M., Raper J. R.: Genetic restriction of energy conservation inSchizophyllum.Science171, 418 (1971).
Kellerman G. M., Biggs D. R., Linnane A. W.: Biogenesis of mitochondria. XI. A comparison of the effects of growth-limiting oxygen tension, intercalating agents, and antibiotics on the obligate aerobeCandida parapsilosis.J. Cell Biol.42, 378 (1969).
Lambowitz A. M., Slayman C. W.: Cyanide-resistant respiration inNeurospora crassa.J. Bacteriol.108, 1087 (1971).
Nešvera J.: Genetic analysis of acetate-nonutilizing mutants ofSchizophyllum commune, Čs. Mykol. in press.
Niederpruem D. J., Hackett D. P.: Cytochrome system inSchizophyllum commune.Plant Physiol.36, 79 (1961).
Raper J. R.: Genetics of sexuality in higher fungi. Ronald Press Co., New York 1966.
Raper J. R., Miles P. G.: The genetics ofSchizophyllum commune.Genetics43, 530 (1958).
Schwab A. J.: Mitochondrial protein synthesis and cyanide-resistant respiration in copper-depleted cytochrome oxidase-deficientNeurospora crassa.FEBS Lett.35, 63 (1973).
Sherald J. L., Sisler H. D.: Antimycin A-resistant respiratory pathway inUstilago maydis andNeurospora sitophila.Plata Physiol.46, 180 (1970).
Sherald J. L., Sisler H. D.: Selective inhibition of antimycin A-insensitive respiration inUstilago maydis andGeratocystis ulmi.Plant Cell Physiol.13, 1039 (1972).
Sherman F., Taber H., Campbell W.: Genetic determination of iso-cytochromesc in yeast.J, Mol. Biol.13, 21 (1965).
Smith D. G., Marchant R.: Chloramphenicol inhibition ofPythium ultimum andRhodotorula glutinis.Arch. Mihrobiol.60, 262 (1968).
Unestam T., Gleason F. H.: Comparative physiology of respiration of aquatic fungi. II. The Saprolegniales, especiallyAphanomyces astaci.Physiol. Plant.21, 573 (1968).
Von Jagow G.,Weiss H., Klingenberg M.: Comparison of the respiratory chain ofNeurospora crassa wild type and the mi-mutants mi-1 and mi-3.Eur. J. Biochem. 33, 140 (1973).
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Nešvbra, J. Cyanide-Insensitive Respiration inSchizophyllum commune . Folia Microbiol 22, 206–215 (1977). https://doi.org/10.1007/BF02885602
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DOI: https://doi.org/10.1007/BF02885602