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Cloning and characterization of a novel member of human β-1,4-galactosyltransferase gene family

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Abstract

By using the EST strategy for identifying novel members belonging to homologous gene families, a novel fulklength cDNA encoding a protein significantly homologous to UDP-Gal: N-acetylglucosamine β-1, 4-galactosyltransferase (GalT) was isolated from a human testis cDNA library. A nucleotide sequence of 2 173 bp long was determined to contain an open reading frame of 1 032 nucleotides (344 amino acids). In view of the homology to memben of the galactosyltransferase gene family and especially the closest relationship toGallus gallus GalT type I (CK I), the predicted product of the novel cDNA was designated as human β-1,4-galactosyltransferase homolog I (HumGT-H1). Its mRNA is present in different degrees in 16 tissues examined. Southern analysis of human genomic DNA revealed its locus on chromosome 3.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, 200433, Shanghai, China

    Yuxin Fan, Long Yu, Qi Zhang, Ying Jiang, Fangyan Dai, Chiyuan Chen, Qiang Tu, Anding Bi, Yuefang Xu & Shouyuan Zhao

Authors
  1. Yuxin Fan
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  2. Long Yu
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  3. Qi Zhang
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  4. Ying Jiang
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  5. Fangyan Dai
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  6. Chiyuan Chen
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  7. Qiang Tu
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  8. Anding Bi
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  9. Yuefang Xu
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  10. Shouyuan Zhao
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Additional information

Poject supported by the 863 High-technology Program, the National Outstanding Young Scientist Foundation and the National Natural Science Foundation of China (Grant No. 39680019).

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Fan, Y., Yu, L., Zhang, Q. et al. Cloning and characterization of a novel member of human β-1,4-galactosyltransferase gene family. Sci. China Ser. C.-Life Sci. 42, 337–345 (1999). https://doi.org/10.1007/BF02882052

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  • DOI: https://doi.org/10.1007/BF02882052

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