Abstract
Upstream regulatory region and flanking DNA ofyellow gene were isolated and cloned from aDrosophila genomic library. A vector containingyellow gene and regulatory elements was constructed using the recombinant DNA technique. Then this vector was integrated intoDrosophila genome by genetic transformation. Using both FLP/FRT and Cre/LoxP site-specific recombination systems, two newyellow alleles were created at the same position in the genome of transgenic flies. Results from genetic and molecular analysis indicated that transcriptional enhancers regulate the developmental expression of the transgene. Furthermore, interactions between new-createdyellow alleles were observed. Such interactions can influence markedly the expression ofyellow gene during development. This effect may also be a form of enhancer-mediated gene expression.
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Geyer, P. K., Green, M. M., Corces, V. G., Tissue-specific transcriptional enhancers may act on the gene located in the homologous chromosome, EMBO J., 1990, 9(7): 2247.
Chen, J. L., Liu, J., Chen, Z. W. et al., Molecular analysis of gene transvection by usingDrosophila yellow gene model, Devel. Reprod. Biol., 1998, 7(2): 43.
Goldsborough, A. S., Kornberg, T. B., Reduction of transcription by homologue asynapsis inDrosophila imaginal discs, Nature, 1996, 381: 807.
Wu, C.-T., Morris, J. R., Transvection and other homology effects, Current Opinion in Genetics & Development, 1999, 9: 237.
Pal-Bhadra, M., Bhadra, U., Birchler, J. A., Cosuppression inDrosophila: gene silencing of alcohol dehydrogenase by white-Adh transgenes is polycomb dependent, Cell, 1997, 90: 479.
Matzke, M. A., Matzke, A. J. M., Homology-dependent gene silencing in transgenic plants: what does it really tell us? Trends Genet., 1995, 11: 1.
Aramayo, R., Metzenberg, R. L., Meiotic transvection in fungi, Cell, 1996, 86: 103.
Leiserson, W. M., Bonini, N. M., Benzer, S., Transvection at theeyes absent gene ofDrosophila, Genetics, 1994, 138: 1171.
Sun, F. L., Dean, W. L., Kelsey, G. et al., Transactivation ofIgf2 in a mouse model of Beckwith-Wiedemann Syndrome, Nature, 1997, 389: 809.
Morris, J. R., Chen, J. L., Geyer, P. K. et al., Two modes of transvection: enhancer action intrans and by pass of a chromatin insulator incis, Proc. Natl. Acad. Sei. USA, 1998, 95: 10740.
Morris, J. R., Chen, J. L., Filandrinos, S. T. et al., An analysis of transvection at theyellow locus ofDrosophila melanogaster, Genetics, 1999, 151: 633.
Chen, J. L., Longo, F. J., Expression and localization of DNA topo II during spermatogenesis, Mol. Reprod. Devel., 1996, 45: 61.
Rubin, G. M., Spradling, A. C., Genetic transformation ofDrosophila with transposable element vectors, Science, 1982, 218: 348.
Johnson, R. C., Site-specific recombinases and their interactions with DNA, in DNA-Protein: Structural Interactions (ed. Lilley, D. M. J.), Oxford: Oxford University Press, 1995, 141–170.
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Chen, J., Liu, J., Chen, Z. et al. Involvement of transcriptional enhancers in the regulation of developmental expression ofyellow gene. Sci. China Ser. C.-Life Sci. 44, 170–177 (2001). https://doi.org/10.1007/BF02879322
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DOI: https://doi.org/10.1007/BF02879322