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Isolation of a (+)- δ-cadinene synthase gene CAD1-A and analysis of its expression pattern in seedlings ofGossypium arboreum L.

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Abstract

The cotton sesquiterpene cyclase, (+)-δ-cadinene synthase, is encoded by a gene family, which can be divided into two subfamilies:CAD1-A and CAD1-C. The geneCAD1-A was isolated fromG. arboreum. In situ hybridization performed on seven-day-old cotton seedlings localized transcripts of both the CAD1 -A and CAD1 -C mainly in lateral root primordium and apical ground meristem, vascular tissues of emerging lateral roots, and also in procambium and some subepidermal cells of the hypocotyl. The CAD1 -A promoter showed a similar tissue-specificity in transgenic tobacco plants. Histochemistry showed occurrence of sesquiterpene aldehydes in outer cells of the lateral root tips, as well as in pigment glands. The CAD1 gene expression in G.arboreum seedlings and the spatial pattern of sesquiterpene biosynthesis constitute a chemical defense machinery in cotton seedlings.

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Authors and Affiliations

  1. National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Shanghai Research Center of Life Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China

    Wanqi Liang, Xiaoping Tan & Xiaoya Chen

  2. Graduate School of Biological Sciences, Nara Institute of Science and Technology, 630-0101, Nara, Japan

    Takashi Hashimoto & Yasuyuki Yamada

  3. Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 47907, West Lafayette, IN, USA

    Peter Heinstein

Authors
  1. Wanqi Liang
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  2. Xiaoping Tan
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  3. Xiaoya Chen
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  4. Takashi Hashimoto
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  5. Yasuyuki Yamada
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  6. Peter Heinstein
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Correspondence to Xiaoya Chen.

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Liang, W., Tan, X., Chen, X. et al. Isolation of a (+)- δ-cadinene synthase gene CAD1-A and analysis of its expression pattern in seedlings ofGossypium arboreum L.. Sci. China Ser. C.-Life Sci. 43, 245–253 (2000). https://doi.org/10.1007/BF02879283

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  • DOI: https://doi.org/10.1007/BF02879283

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