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Assessment of oxidative stress and antioxidant status in age related cataract in a rural population

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Abstract

Oxidative stress was assesed by estimating lipid peroxidation product (LPO) in the form of thiobarbituric acid reactive substances (TBARS), enzymatic antioxidants in the form of superoxide dismutase (SOD), catalase and nonenzymatic antioxidant vitamins e.g. vitamin C, β carotene and vitamin E in either serum or plasma or erythrocytes in 190 cases of age related cataract in the age group of 50–80 years. 190 cases were grouped into three morphological types namely, 73 cases of cortical, 77 cases of posterior subcapsular and 40 cases of nuclear cataract and values of LPO and antioxidants were compared with 78 cases of age matched healthy control groups. Plasma TBARS levels were cataract cases when compared with control groups. There were no significant differences in the erythrocyte levels of catalase and plasma levels of Vit E between cataract cases and control groups. No significant changes of parameters were seen among three different morphological types of age related cataract. The present study shows that the oxidative stress may play an important role in the age related cataract.

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References

  1. Venkatesh, P., Garg, S., Verma, L., Tewari and Garg, S. (2001) Antioxidants: Basic Concepts in relation to the eye Ind. J. Clin. Biochem. 16(1), 9–14

    Article  CAS  Google Scholar 

  2. Irashad, M. and Chaudhuri, P.S. (2002) Oxidant —antioxidant System: Role and significance in human body. Ind. J. Exp. Biol. 40, 1233–1239

    Google Scholar 

  3. Spector, A. (1995) Oxidative stress—induced cataract: mechanism of action. FASEB. J. 9, 1173–1182

    PubMed  CAS  Google Scholar 

  4. Jacques, P.F., Chylack, Jr., Leo, T., McGandy, R.B. and Hartz, S.C. (1988) Antioxidant status in persons with and without senile cataract. Arch. Ophthalmol. 106, 337–340.

    Article  PubMed  CAS  Google Scholar 

  5. Mohan, M., Sperduto, R.D., Angra, S.K., Milton, R.C., Mathur, R.L., Underwood, B.A., Jaffery, N., Pandya, C.B., Chhabra, V.K., Vajpayee, R.B., Kalra, V.K. and Sharma, Y.R. (1989) India—US case control study of age related cataracts. Arch. Ophthalmol. 107, 670–676.

    PubMed  CAS  Google Scholar 

  6. Garg, R., Verma, M.,Mathur, S. P. and Murthy, P.S. (1996) Blood lipid peroxidation products and antioxidants in senile cataract. Ind. J. Clin. Biochem. 11(2), 182–186.

    CAS  Google Scholar 

  7. Knekt, P., Heliovaara, M., Rissanen, A., Aromaa, A., and Aaran, R.K. (1992) Serum antioxidant vitamins and risks of cataract. B.M.J., 305, 1392–4.

    CAS  Google Scholar 

  8. Gale, C.R., Hall, N.F., Phillips, D.I.W. and Martyn, C.N. (2001) Plasma antioxidant vitamins and carotenoids and age related cataract. Ophthalmology 108, 1992–1998

    Article  PubMed  CAS  Google Scholar 

  9. Donma, O., Yorulmaz, E., Pekel, H.and Syugul, N. (2002) Blood and lens lipid peroxidation and antioxidant status in normal individuals, senile and diabetic cataractous patients. Curr. Eye Res.25(1), 9–16.

    Article  PubMed  Google Scholar 

  10. Olmedilla, B., Granado, F., Blanco, I., Herrero, C., Vaquero, M. and Millan, I. (2002) Serum status of carotenoids and tocopherols in patients with age related cataracts: a case control study. J. Nutr.Health Aging 6(1), 66–68.

    PubMed  CAS  Google Scholar 

  11. Satoh, K. (1978) Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin. Chim. Acta 90, 37–43.

    Article  PubMed  CAS  Google Scholar 

  12. Marklund, S. and Marklund, G. (1974) Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469–474.

    Article  PubMed  CAS  Google Scholar 

  13. Sinha, A.K. (1972) Colorimetric assay of catalase. Analytical Biochemistry 47, 389–394.

    Article  PubMed  CAS  Google Scholar 

  14. Tietz, N.W. (1994) Textbook of clinical chemistry, 2nd edn, Eds. Carl A. Burtis and Edward R. Ashwood. W.B. Saunders Company, Philadelphia, London, Toronto, Montreal, Sydney, Tokyo p. 1313–1314.

    Google Scholar 

  15. Bradley, D. W. and Hornbeck, C. L. (1973) A Clinical evaluation of an improved TFA micromethod for plasma and serum Vitamin A. Biochem. Med. 7, 78–86.

    Article  PubMed  CAS  Google Scholar 

  16. Bieri, J.G., Teets, L., Belavady, B. and Andrews, E.L. (1964) Serum vitamin E levels in a normal adult population in the Washington DC area. Proc. Soc. Exptl. Biol. Med. 117, 131–133.

    CAS  Google Scholar 

  17. Babizhayev, M.A. (1989) Accumulation of lipid peroxidation products in human cataracts. Acta Ophthalmol. 67, 281–287.

    Article  CAS  Google Scholar 

  18. Bhuyan, K.C. and Bhuyan, D.K. (1978) Superoxide dismutase of the eye. Relative functions of superoxide dismutase and catalase in protecting the ocular lens from oxidative damage. B.B.A. 542 28–38.

    CAS  Google Scholar 

  19. Casado, A., De La Torre, R. and Lopez-Femandez, E. (2001) Antioxidant enzyme levels in red blood cells from cataract patients. Gerontology 47(4), 186–188.

    Article  PubMed  CAS  Google Scholar 

  20. Fecondo, J.V. and Augusteyn, R.C.(1983) Superoxide dismutase, Catalase and Glutathione peroxidase in the human cataractous lens. Exp. Eye Res. 36, 15–23

    Article  PubMed  CAS  Google Scholar 

  21. Ray, G., and Husain, S.A. (2002) Oxidants, antioxidants and carcinogenesis. Ind. J. Exp. Biol. 40, 1213–1232.

    CAS  Google Scholar 

  22. Jacques, P.F., Hartz, S.C., Chylack, Jr. L. T., McGandy, R.B. and Sadowski, J.A. (1988) Nutritional status in persons with and without senile cataract: blood vitamin and mineral levels. Am. J. Clin. Nutr. 48, 152–158.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and J.B. Tropical Disease Research Centre, MGIMS, 442 102, Wardha, Sevagram, India

    A. K. Pradhan, M. V. R. Reddy & N. Garg

  2. Department of Ophthalmology, MGIMS, 442 102, Sevagram, Wardha, India

    A. K. Shukla

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  1. A. K. Pradhan
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  2. A. K. Shukla
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  3. M. V. R. Reddy
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  4. N. Garg
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Pradhan, A.K., Shukla, A.K., Reddy, M.V.R. et al. Assessment of oxidative stress and antioxidant status in age related cataract in a rural population. Indian J Clin Biochem 19, 83–87 (2004). https://doi.org/10.1007/BF02872397

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