Abstract
The results of serological typing of root-nodule homogenates from soybean plants inoculated by mixed bacterial inocula under field and greenhouse conditions are submitted. The inocula were prepared from strains capable of serological differentiation on the basis of their somatic antigens. Individual root-nodule homogenates were typed serologically by immunoprecipitation in agar with antisera against the inoculum strains. This method gave accurate determination of the origin of 605 out of a total of 616 serologically typed root-nodules and of the individual bacterial strains which participated in their formation.
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Damirgi, S. M., Frederick, L. R., Anderson, I. C.:Serogroups of Rhizobium japonicum in soybean nodules as affected by soil types. Agron. J. 59: 10, 1967.
Holland, A. A.:Serologic characteristic of certain root-nodule bacteria of legumes. Antonie van Leeuwenhoek 32: 410, 1966.
Izrailsky, V. P., Ryzhova, A. S.:Application of the serological method for selection of competitive strains of nodule bacteria under laboratory conditions. Izv. Akad. Nauk. SSSR Ser. biol. 4: 567, 1967.
Johnson, H. W., Means, U. M., Weber, C. R.:Competition for nodule sites between strains of Rhizobium japonicum applied as inoculum and strains in the soil. Agron. J. 57: 179, 1965.
Loos, M. A.:Serological characteristics of clover rhizobia from the George District, Cape Province. S. Afr. J. Lab. Clin. Med. (Sept.): 124, 1962.
Loos, M. A., Louw, H. A.:A study of the clover root nodule bacteria in soils of the George District. I. Serological characteristics. S. Afr. J. Agric. Sci. 7: 135, 1964.
Means, U. M., Johnson, H. W., Date, R. A.:Quick serological methods of classifying strains of Rhizobium japonicum in nodules. J. Bacteriol. 87: 547, 1964.
Ouchterlony, O.:Diffusion-in-gel methods for immunological analysis. Progr. Allergy 5: 1, 1958.
Scheffler, J. G., Louw, H. A.:The gel immune diffusion technique as a method for studying the antigen relationships among rhizobia. S. Afr. Med. J. 40: 701, 1966.
Scheffler, J. G., Louw, H. A.:The serological characteristics of the clover rhizobia in the soils of the Stellenbosch District. S. Afr. J. Agric. Sci. 10: 161, 1967.
Škrdleta, V.:The serological identity of agar cultures of Rhizobium japonicum and bacterial component of soybean root-nodules. (In Czech) Rostl. výroba 13: 671, 1967.
Škrdleta, V.:Determination of the soybean rootnodule origin by the Ouchterlony method. Zbl. Bakt. II. Abt., 123, 1969.
Thornton, H. G., Kleczkowski J.:Use of antisera to identify nodules produced by the inoculation of legumes in the field. Nature 166: 1118, 1950.
Vincent, I. M.:The root-nodule bacteria of pasture legumes. Proc. Linn. Soc. N. S. W. 79: 1, 1954.
Vincent, I. M., Waters, L. M.:The influence of the host on competition among clover root-nodule bacteria. J. gen. Microbiol. 9: 357, 1953.
Vintiková, H., Šrogl, M., Škrdleta, V.:A contribution to the serological typization of the Rhizobia. Fol. microbiol. 6: 243, 1961.
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Škrdleta, V. Application of immunoprecipitation in agar gel for the serological typing of soybean root-nodules. Folia Microbiol 14, 32–35 (1969). https://doi.org/10.1007/BF02869395
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DOI: https://doi.org/10.1007/BF02869395