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Introgression between wild and cultivated soybeans of Japan revealed by RFLP analysis for chloroplast DNAs

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Abstract

Wild soybeans collected in Japan were surveyed for RFLPs of chloroplast DNA. Three haplotypes were detected in RFLPs with a cpDNA clone which contains a LSC region adjacent to the left member of IR. Most of the plants tested possessed haplotype III, and a few plants, collected mostly in southern Japan, had haplotype II. Haplotype I, which is the predominant form in modern cultivars, was detected at six sites from four widely separated regions. Our results indicate that haplotype III is predominant in wild soybean of Japan. Some of the plants having haplotype I were phenotypically intermediate between wild and cultivated soybeans, while the others possessed a seed morphology and plant architecture typical of ordinary wild soybean. The plants having haplotype I appear to be either derivatives of hybridization between wild and cultivated soybeans or relics of a direct progenitor of soybean cultivars with the haplotype I chloroplast genome.

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Literature Cited

  • Beebe, S., O. Ch. Toro, A. V. Gonzàlez, M. I. Chacòn, and D. G. Debouck. 1997. Wild-weed-crop complexes of common bean (Phaseolus vulgaris L., Fabaceae) in the Andes of Peru and Colombia, and their implications for conservation and breeding. Genetic Resources and Crop Evolution 44:73–91.

    Article  Google Scholar 

  • Broich, S., and R. G. Palmer. 1980. A cluster analysis of wild and domesticated soybean phenotypes. Euphytica 29:23–32.

    Article  Google Scholar 

  • Close, P. S., R. C. Shoemaker, and P. Keim. 1989. Distribution of restriction site polymorphism within the chloroplast genome of the genusGlycine sub-genusSoja. Theoretical and Applied Genetics 77: 768–776.

    Article  CAS  Google Scholar 

  • de Wet, J. M. J., and J. R. Harlan. 1975. Weeds and domesticates: evolution in the man–made habitat. Economic Botany 29:99–107.

    Google Scholar 

  • Doyle, J. J., and J. L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13–15.

    Google Scholar 

  • Fujita, R., M. Ohara, K. Okazaki, and Y. Shimamoto. 1997. The extent of natural cross–pollination wild soybean (Glycine soja). Journal of Heredity 88:124–128.

    Google Scholar 

  • Fukuda, Y. 1933. Cytological studies on the wild and cultivated Manchurian soybeans. Japanese Journal of Botany 6:489–506.

    Google Scholar 

  • Hatfleld, P. M., R. C. Shoemaker, and R. G. Palmer. 1985. Maternal inheritance of chloroplast DNA within the genusGlycine, subgenussoja. Journal of Heredity 76:373–374.

    Google Scholar 

  • Hymowitz, T. 1970. On the domestication of the soybean. Economic Botany 24:408–421.

    Google Scholar 

  • —,and R. J. Singh. 1987. Taxonomy and speciation. Pages 23–48in J. R. Wilcox, ed., Soybean: improvement, production, and uses. 2nd ed. American Society of Agronomy, Madison, Wisconsin.

    Google Scholar 

  • —,and C. A. Newell. 1980. Taxonomy, speciation, domestication, dissemination, germplasm resources and variation in the genusGlycine. Pages 251–264in R. J. Summerfield and A. H. Bunting, eds., Advances in legume science. Royal Botanic Garden, Kew, UK.

    Google Scholar 

  • Johns, T., Z. Huamàn, C. Ochoa, and P. E. Schmiediche. 1987. Relationships among wild, weed, and cultivated potatoes in theSolanum xajanhuiri complex. Systematic Botany 12:541–552.

    Article  Google Scholar 

  • Kanazawa, A., A. Tozuka, and Y. Shimamoto. 1998. Sequence variation of chloroplast DNA that involvesEcoRl andClal restriction site polymorphisms in soybean. Genes and Genetic Systems 73: 111–119.

    Article  PubMed  CAS  Google Scholar 

  • Kiang, Y. T., and Y. C. Chiang. 1989. Latitudinal variation and evolution in wild soybean (Glycine soja Sieb. & Zucc.) populations. Pages 469–468in J. H. Bock and Y. B. Linhart, eds., The evolutionary ecology of plants. Westview Press, Boulder, Colorado.

    Google Scholar 

  • Kirkpatrick, K. J., and H. D. Wilson. 1988. Interspecific gene flow in Cucurbita:C. texana vs.C. pepo. American Journal of Botany 75:519–527.

    Article  Google Scholar 

  • Kishima, Y., T. Mikami, T. Harada, K. Shinozaki, M. Sugiura, and T. Kinoshita. 1986. Restriction fragment map of sugar beet (Beta vulgais L.) chloroplast DNA. Plant Molecular Biology 7:201–205.

    Article  CAS  Google Scholar 

  • Kwon, S. H. 1972. History and the land races of Korean soybean. SABRAO Newsletter 4:107–111.

    Google Scholar 

  • Oka, H. I. 1983. Genetic control of regenerating success in semi-natural conditions observed among lines derived from a cultivated x wild soybean hybrid. Journal of Applied Ecology 20:937–949.

    Article  Google Scholar 

  • Palmer, J. D. 1987. Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. American Naturalist Supplement 130:S74-S100.

    Google Scholar 

  • — 1990. Contrasting modes and tempos of genome evolution in land plant organelles. Trends in Genetics 6:115–120.

    Article  PubMed  CAS  Google Scholar 

  • —,G. P. Singh, and D. T. N. Pillay. 1983. Structure and sequence evolution of three legume chloroplast DNAs. Molecular and General Genetics 190:13–19.

    Article  CAS  Google Scholar 

  • —,B. Osorio, and W. F. Thompson. 1988. Evolutionary significance of inversions in legume chloroplast DNAs. Current Genetics 14:65–74.

    Article  CAS  Google Scholar 

  • Palmer, R. G., and H. E. Heer. 1984. Agronomic characteristics and genetics of a chromosome interchange in soybean. Euphytica 33:651–663.

    Article  Google Scholar 

  • —,K. E. Newhouse, R. A. Graybosch, and X. Delannay. 1987. Chromosome structure of the wild soybean. Journal of Heredity 78:243–247.

    Google Scholar 

  • Sekizuka, S., and T. Yoshiyama. 1960. Studies on the native wild grasses for fodder. IV. Crop-scientific studies on wild species ofGlycine soja in Japan. Journal of Kantou-Tosan Agricultural Experimental Station 15:57–73.

    Google Scholar 

  • Shoemaker, R. C, P. M. Hatfleld, R. G. Palmer, and A. G. Atherly. 1986. Chloroplast DNA variation in the genusGlycine subgenusSoja. Journal of Heredity 77:26–30.

    CAS  Google Scholar 

  • Skvortzow, B. V. 1927. The soybean-wild and cultivated in Eastern Asia. Manchurian Research Society Publication, Service A Natural History Section 22:1–8.

    Google Scholar 

  • Spielmann, A., W. Ortiz, and E. Stutz. 1983. The soybean chloroplast genome: construction of a circular restriction site map and location of DNA regions encoding the genes for rRNAs, the large subunit of the ribulose-l,5-bisphosphate carboxylase and the 32 KD protein of the photosystem II reaction center. Molecular and General Genetics 190: 5–12.

    Article  CAS  Google Scholar 

  • Suh, H. S., Y. I. Sato, and H. Morishima. 1997. Genetic characterization of weedy rice (Oryza sativa L.) based on morpho-physiology, isozymes and RAPD markers. Theoretical and Applied Genetics 94:316–321.

    Article  CAS  Google Scholar 

  • Yamaguchi, H. 1992. Wild and weed azuki beans in Japan. Economic Botany 46:384–394.

    Google Scholar 

  • —,and S. Umemoto. 1997. Wild relatives of domesticated plants as genetic resources in disturbed environments in temperature East Asia: a review. Applied Biological Science 2:1–16.

    Google Scholar 

  • Wilkes, H. G. 1977. Hybridization of maize and teosinte in Mexico and Guatemala and the improvement of maize. Economic Botany 31:254–293.

    Google Scholar 

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Abe, J., Hasegawa, A., Fukushi, H. et al. Introgression between wild and cultivated soybeans of Japan revealed by RFLP analysis for chloroplast DNAs. Econ Bot 53, 285–291 (1999). https://doi.org/10.1007/BF02866640

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