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Assay of tubercular antibody, circulating free and immune complexed antigen in the diagnosis of pulmonary tuberculosis

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Abstract

Analysis of tubercular antibody, circulating free and immune complexed antigen (CIC-Ag) was done in confimed pulmonary tuberculosis sera by ELISA, using ES-31 antigen and affinity purified anti ES-31 antibody. Twenty three of 25 (92%) tuberculosis sera were positive for IgG antibody to ES-31 antigen. Using anti ES-31 antibody, free tubercular antigen could be detected in 20 of 25 (80%) cases whereas circulating immune complexed antigen (CIC-Ag) in 18 of 25 (72%) cases by sandwich ELISA. Of the two sera showing absence of antibody, one showed presence of free and CIC-Ag whereas the other showed the presence of free antigen. Thus antigen assay may be used as an adjunct tool for confirmation of pulmonary tuberculosis.

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Authors and Affiliations

  1. Jamnalal Bajaj Tropical Disease Research Centre and Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, 442 102, Sevagram, Wardha, Maharashtra, India

    Swati Banerjee, E Raji Nair, Satish Kumar, M. V. R. Reddy & Bhaskar C. Harinath (Director Professor and Head)

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  1. Swati Banerjee
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  2. E Raji Nair
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  3. Satish Kumar
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  4. M. V. R. Reddy
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  5. Bhaskar C. Harinath
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Correspondence to Bhaskar C. Harinath.

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Banerjee, S., Nair, E.R., Kumar, S. et al. Assay of tubercular antibody, circulating free and immune complexed antigen in the diagnosis of pulmonary tuberculosis. Indian J Clin Biochem 16, 203–206 (2001). https://doi.org/10.1007/BF02864862

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