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Evaluation of a mass inoculation method of potatoes withPseudomonas solanacearum

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Abstract

Wounding of potato stems by the application of clean sand at a 45° angle at 550 kPa pressure followed by a spray-mist ofPseudomonas solanacearum proved to be the most reliable method of rapidly inoculating a large quantity of potato plants to incite bacterial wilt in the field. The technique was 100% efficient (320/320) and it was estimated that 10,000 plants/hr could be inoculated by using this method.

Because of its hygroscopic nature, sand coated withP. solanacearum created too many mechanical problems to be of practical value for field use even though the amount of wilt after 21 days was not significantly different from that of the best treatment. In addition, viability of inoculum as a bacterial suspension in water was greater than as coated sand.

Resumen

El herir tallos de plantas de papa aplicando arena limpia en un ángulo de 45° con una presión de 550 kPa y a continuación rociarlo conPseudomonas solanacearum, mostró ser el método más confiable de inocular rápidamente una gran cantidad de plantas de papa para provocar marchitez bacteriana en el campo. La técnica fue 100% eficiente (320/320) y se estimó que se podian inocular 10,000 plantas/hora con este método.

Debido a su naturaleza higroscópica, la arena tratada conP. solanacearum creó demasiados problemas mecánicos para tener algún valor práctico en su utilización en el campo aunque la cantidad de marchitez, después de 21 días, no fue significativamente diferente de la del mejor tratamiento. Además, la viabilidad del inóculo en la forma de una suspensión bacteriana en agua fue mayor que como arena recubierta.

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Gitaitis, R.D., Ghate, S.R., Jaworski, C.A. et al. Evaluation of a mass inoculation method of potatoes withPseudomonas solanacearum . American Potato Journal 60, 625–630 (1983). https://doi.org/10.1007/BF02854112

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