Abstract
Twenty-three diploid (2n=2x=24) potato clones were screened for response to four different anther culture media. Anther derived tissues (calli/embryos) were obtained from seven of the clones in liquid culture media. Anther culture response of these seven clones was further quantified on five additional media. Since clonal responses are specific to culture conditions (Tiainen, 1992), the seven genotypes somewhat differed from each other in the magnitude of the response to each of the media. In agreement with Heberle-Bors (1985) a more realistic assessment of the responsiveness was possible when pollen counts per anther were used to express the yield of anther-derived material. A relatively higher level of exogenous cytokinin (6-benzyladenine) to that of auxin (3-indoleacetic acid) proved to be favourable for the inducton of calli/embryos. Use of a semi-solid medium saved space and was convenient for culture observations therefore it was found more conducive for the genotypic screening. Anther derived tissues that regenerated to produce root tissue and/or plantlets were further multiplied on a root culture medium and a plantlet medium. Liquid culture conditions on orbital shakers were conducive for the growth of root explants. Anther derived material (roots and plantlets) originating from clone 9507–04 revealed differences in growth vigor. Flow cytometric estimation of ploidy in regenerated roots and plantlets, revealed that clone 9507–04 produced 44% monoploid roots and 77% monoploid plantlets. Seventy-three percent anther-derived plantlets from clone 6028–02 were monoploids.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Calleberg, E. 1996. Anther culture of potato. Doctoral Dissertation. Swedish University of Agricultural Sciences, Uppsala, Sweden. 59 pp.
Heberle-Bors, E. 1985. In vitro monoploid formation from pollen: a critical review. Theor Appl Genet 71:361–374.
Hougas, R.W., S.J. Peloquin, and A.C. Gabert. 1964. Effect of seed parent and pollinator on the frequency of monoploids inSolanum tuberosum. Crop Sci 4:593–595.
Janjua, S. 1988. Anther culture in selected lines of diploidSolanum tuberosum L., relatives and interspecific hybrids. M. Sc. Thesis. Département de sciences biologiques. Université de Montréal, 90 pp.
Martin, B. and J.M. Widholm. 1996. Ploidy of small individual embryolike structures from maize anther cultures treated with chromosome doubling agents and calli derived from them. Plant Cell Rep 15:781–785.
Meyer, R., F. Salamini, and H. Uhrig. 1993. Isolation and characterization of potato diploid clones generating a high frequency of monomonoploid or homozygous diploid androgenetic plants. Theor Appl Genet 85:905–912.
Mozafari, J., S.T. Ali-Khan,.D.J. Wolyn, and R.R. Martin. 1995. Genetic engineering of potato chipping cultivais for PLRV resistance. Am Potato J 72:642–643 (Abstr.).
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497.
Pijnacker, L.P., K. Sree Ramulu, P. Dijkhuis, and M.A. Ferwerda, 1989. Flow cytometric and karyological analysis of polysomaty and polyploidization during callus formation from leaf segments of various potato genotypes. Theor Appl Genet 77:102–110.
Ríhová, L. and J. Tupy. 1996. Influence of 2,4-D and lactose on pollen embryogenesis in anther culture of potato. Plant Cell Tiss Org Cult 45:269–272.
Rivard, S.R., M. Cappadocia and B.S. Landry. 1996. A comparison of RFLP maps based on anther culture derived, selfed, and hybrid progeniesof Solanum chacoense. Genome 39:611–621.
Rokka, V. -M., J.P.T. Valkonen, and E. Pehu. 1995. Production and characterization of monoploids derived from somatic hybrids betweenSolanum brevidens andS. tuberosum through anther culture. Plant Sci 112:85–95.
Seabrook, J.E.A. 1990. Optimizing the propagation of potato (Solanum tuberosum L.) by stem cuttings. Am Potato J 67:267–275.
Shen, L.Y. and R.E. Veilleux. 1995a Yield of embryos from sequential anther culture. Am Potato J 72:689–700.
Shen, L.Y. and R.E. Veilleux. 1995b. Effect of temperature shock and elevated incubation temperature on androgenic embryo yield of diploid potato. Plant Cell Tiss Org Cult 43:29–35.
Tiainen, T. 1992. The influence of culture conditions on anther culture response of commercial varieties ofSolanum tuberosum L. Plant Cell Tiss Org Cult 30:211–219.
Tiainen, T. 1993. The influence of hormones on anther culture response of tetraploid potato (Solanum tuberosum L.) Plant Sci 88:83–90.
Uhrig, H. 1985. Genetic selection and liquid medium conditions improve the yield of androgenetic plants from diploid potatoes. Theor Appl Genet 71:455–460.
Uijtewaal, B.A., D.J. Huigen, and J.G. Th. Hermen. 1987. Production of monomonoploid (2n=x=12) through prickle pollination. Theor Appl Genet 73:751–758.
Veilleux, R.E., L.Y. Shen and M.M. Paz. 1995. Analysis of the genetic composition of anther-derived potato by randomly amplified polymorphic DNA and simple sequence repeats. Genome 38:1153–1162.
White, P.R. 1963. The cultivation of animal and plant cells. 2nd edn. Ronald Press Co., New York, USA, 228 pp.
Xu, Y., L. Zhu, J. Xiao, N. Huang, and S.R. McCouch. 1997. Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, double monoploid, and recombinant inbred populations in rice (Oryza sativa L.). Mol Gen Genet. 253:535–545.
Yamagishi, M., M. Yano, Y. Fukuta, K. Fukui, M. Otani, and T. Shimada. 1996. Distorted segregation of RFLP markers in regenerated plants derived from anther culture of an F1 hybrid of rice. Genes Genet Syst 71:37–41.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Aziz, A.N., Seabrook, J.E.A., Tai, G.C.C. et al. Screening diploidSolarium genotypes responsive to different Anther culture conditions and ploidy assessment of Anther-derived roots and plantlets. Am. J. Pot Res 76, 9–16 (1999). https://doi.org/10.1007/BF02853552
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02853552