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Genetic relationships of Japanese potato cultivars assessed by RAPD analysis

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Abstract

Seventy-three breeding lines and cultivars, which included all Japanese cultivars, were assessed by random amplified polymorphic DNA (RAPD) analysis. Thirty-one of 40 decamer primers revealed 84 scorable RAPDs. Using these RAPDs, 67 of 73 cultivars were distinguished from each other. One of the cultivars released as a protoclonal variant was able to distinguish from the parent by increasing the number of primers surveyed. Based on the number of different RAPDs between each pair of accessions, cluster analysis was performed to exploit genetic diversity in Japanese cultivars. Closely related cultivars deduced from their pedigrees were clustered closely, indicating that similarities based on the presence vs. absence of RAPDs were reflections of genetic similarities. Old Japanese cultivars, as suggested by their similarity of chloroplast DNA toS. tuberosum ssp.andigena, were indeed clustered with ssp.andigena accessions, supporting these were relic cultivars of early European potatoes. The present study suggests that Japanese potatoes are an interesting mass of genetic diversity including North American, European, and Andean germplasms and relic potatoes.

Compendio

Setenta y tres líneas de mejoramiento y cultivares, que incluyen a todos los cultivares japoneses, fueron determinados por el análisis del DNA polimórfico amplificado al azar (RAPD). Treinta y uno de 40 imprimadores decameros revelaron 84 RAPDs posibles de marcar. Utilizando estos RAPDs, 67 de 73 cultivares fueron distinguidos unos de los otros. Uno de los cultivares liberados como una variante protoclonal fue distinguido del progenitor incrementando el numéro de imprimadores estudiados. Basándose en el numéro de diferentes RAPDs entre cada par de entradas, se llevó a cabo un análisis de grupos para aprovechar la diversidad genética en los cultivares japoneses. Los cultivares muy relacionados a juzgar por sus genealogías se encontraban bien agrupados, indicando que las similitudes basadas en la presencia o ausencia de RAPDs eran un reflejo de las similitudes genéticas. Los viejos cultivares japoneses, a juzgar por la similitud del DNA de sus cloroplastos a S. tuberosum ssp. andigena, estaban de hecho agrupados con las entradas de la ssp. andigena, apoyadas por los viejos cultivares de las primeras papas europeas. Este estudio sugiere que las papas japonesas son una masa imteresante de diversidad genética incluyendo germoplasmas norteamericanos, europeos y andinos, y papas muy antiguas.

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Authors and Affiliations

  1. Experimental Farm, Kobe University, 1348 Uzurano, Kasai, 675-21, Hyogo, Japan

    K. Hosaka

  2. Laboratory of Potato Breeding, Hokkaido National Agricultural Experiment Station, 829 Shimo-Shimamatsu, Eniwa, 061-13, Hokkaido, Japan

    M. Mori

  3. Tokachi Potato Research Center, Calbee Potato Inc., 3-23 Minami, Higashi-Memuro, Memuro, 082, Hokkaido, Japan

    K. Ogawa

Authors
  1. K. Hosaka
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  2. M. Mori
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  3. K. Ogawa
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Hosaka, K., Mori, M. & Ogawa, K. Genetic relationships of Japanese potato cultivars assessed by RAPD analysis. American Potato Journal 71, 535–546 (1994). https://doi.org/10.1007/BF02851325

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  • DOI: https://doi.org/10.1007/BF02851325

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