Abstract
Hexaploid somatic hybrids have been obtained by fusion of protoplasts fromSolanum brevidens (PI 218228, 2x = 2n = 24) andS. tuberosum (PI 203900 or cv. Russet Burbank; 2x = 4n = 48). In the work reported here, pentaploid progeny derived from sexual crosses between the somatic hybrids and the potato cultivar, Katahdin were assessed for transfer of disease resistances and improvement of agronomic traits. Segregation was noted in disease resistances to race 0 ofPhytophthora infestans and to tuber soft rot caused byErwinia spp., indicating that sexual transfer of traits captured by protoplast fusion had occurred. The pentaploid progeny showed substantial variation in and often improvement of some agronomic traits as compared to the somatic hybrids. The results indicate that interspecific somatic hybridization can produce material with potential for use in a potato breeding program
Compendio
Se han obtenido híbridos somáticos hexaploides por fusión de protoplastos deSolanum brevidens (PI218228, 2x = 2n = 24)yS. tuberosum (PI203900 o cv Russet Burbank; 2x = 4n = 48). En el trabajo, cuyo informe se presenta aquí, las progenies pentaploides se obtuvieron de cruzamientos sexuales entre los híbridos somáticos y el cultivar de papa Katahdin y se evaluaron para transferencia de resistencias a enfermedades y el mejoramiento de atributos agronómicos. Se notó segregación en las resistencias a enfermedades, a la raza 0 dePhytophthora infestans y a la pudrición suave de los tubérculos causada porErwinia spp., indicando que la transferencia sexual de atributos capturada al usar fusión de protoplastos había ocurrido. En comparación con los híbridos somáticos, la progenie pentaploide mostró una variatión sustancial, y frecuentemente, la mejora de algunos atributos agronómicos. Los resultados indican que la hibridación somática interespecífica puede producir material con potencial para ser utilizado en un programa de mejoramiento de papa.
Similar content being viewed by others
Literature Cited
Austin, S., M. Baer and J.P. Helgeson. 1985. Transfer of resistance to potato leaf roll virus fromSolanum brevidens intoSolanum tuberosum by somatic fashion. Plant Science 39:75–82.
Austin, S., M. Baer, M. Ehlenfeldt, P.J. Kazmierczak and J.P. Helgeson. 1985. Intraspecific fusions inSolanum tuberosum. Theor Appl Genet 71:172–176.
Austin, S., M. Ehlenfeldt, M. Baer and J.P. Helgeson. 1986. Somatic hybrids produced by protoplast fusion betweenS. tuberosum andS. brevidens: phenotypic variation under field conditions. Theor Appl Genet 71:682–690.
Austin S. and J.P. Helgeson. 1987. Interspecific somatic fusions betweenSolanum brevidens andS. tuberosum.In: Plant Molecular Biology, pages 209–222, Edited by D. von Wettsein and Nam-Ha i Chua, Eds. Plenum Publishing Corporation, NY, pp. 209–222.
Austin, S., E. Lojkowska, M.K. Ehlenfeldt, A. Kelman and J.P. Helgeson. 1988. Fertile interspecific hybrids of Solanum: A novel source of resistance to Erwinia soft rot. Phytopathology 78:1216–1220.
Barsby, T., J. Shepard, R. Kemble and R. Wong. 1984. Somatic hybridization in the genusSolanum:S. tuberosum andS. brevidens. Plant Cell Reports 3:165–167.
Butenko, R. and A. Kuchko. 1979. Somatic hybridization ofSolanum tuberosum L. andSolanum chacoense Bitt. by protoplast fusion.In: Advances in Protoplast Research, Akad. Kiado, Budapest pages 293–297.
Debnath, S.C. and G. Wenzel. 1987. Selection of somatic fusion products in potato by hybrid vigour. Potato Research 30:371–380.
Ehlenfeldt, M.K. and J.P. Helgeson. 1987. Fertility of somatic hybrids from protoplast fusions ofSolanum brevidens andS. tuberosum. Theor Appl Genet 73:395–402.
Fish, N., A. Karp and M.K. Jones. 1987. Improved isolation of DihaploidSolanum tuberosum protoplasts and the production of somatic hybrids between dihaploidsS. tuberosum andS. brevidens. In vitro Cellular and Deb Biol 23:575–580.
Haberlach, G.T., B.A. Cohen, N.A. Reichert, M.A. Baer, L.E. Towill and J.P. Helgeson. 1985. Isolation, culture and regeneration of protoplasts from potato and several relatedSolanum species. Plant Science 39:67–74.
Helgeson, J.P., G.J. Hunt, G.T. Haberlach and S. Austin. 1986. Somatic hybrids betweenSolanum brevidens andSolanum tuberosum: Expression of a late blight resistance gene and potato leaf roll resistance. Plant Cell Reports 3:212–214.
Melchers, G., M.D. Sacristan and A. Holder. 1978. Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Research Com. 43:203–218.
Puite, K.J., S. Roest and L.P. Pijnacker. 1986. Somatic hybrid potato plants after electrofusion of diploidSolanum tuberosum andSolanum phureja. Plant Cell Reports 5:262–264.
Shepard, J., D. Bidney, T. Barsby and R. Kemble. 1983. Genetic transfer in plants through interspecific protoplast fusion. Science 219:683.
Williams, C.E., G.T. Hunt and J.P. Helgeson. 1990. Fertile somatic hybrids ofSolanum species: RFLP analysis of a hybrid and its sexual progeny with crosses with potato. Theor Appl Genet 80:545–551.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Helgeson, J.P., Haberlach, G.T., Ehlenfeldt, M.K. et al. Sexual progeny of somatic hybrids between potato andSolanum brevidens: Potential for use in breeding programs. American Potato Journal 70, 437–452 (1993). https://doi.org/10.1007/BF02849063
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02849063