Abstract
An alternative pretreatment method for mitotic chromosomes in potato root tips is described. Root tips were treated with a synthetic pyrethroid, a permethrine, {3-Phenoxybenzil (±) -Cis, trans, 3-(2,2 dichlorovinyl) -2,2-dimethyl ciclopropane carboxylate}. with concentrations of 10ppm, 100ppm, 200ppm, and 500ppm. Cold water and 0.02M 8-hydroxyquinoline were used for comparison at 4 C. Mitotic index was obtained for all treatments by using a standard squash method, and pretreatment with 100ppm of the pyrethroid demonstrated a large number of mitotic cells and well condensed chromosomes for a comprehensive observation in mitotic cells
Compendio
Se presenta una tecnica alternativa para tratar chromosomas mitoticos como pretratamiento en raices de papas. Raices se trataron con pyrethroides syntheticos, una permethrina, (carboxilato ciclopropano-3- Fenoxibencilo (±)Cis trans-2,2-dimetilo 3-(2,2-diclorovinila) en concentracion de 10ppm, 100ppm, 200ppm, y 500ppm. Agua helada y 8-hydroxyquinolina 0.02M se emplearon como controles para comparar los diferentes tratamientos con pyrethroides a una temperatura de 4C. El indice mitotico se determinó por el method microscopico establecído para preparar muestras de puntas de raices. Con el pretratamiento con 100ppm de pyrethroide a 100ppm se encontró un gran numero de celulas mitoticas y chromosomas bien observables.
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Watanabe, K.N., Orrillo, M. An alternative pretreatment method for mitotic chromosome observation in potatoes. American Potato Journal 70, 543–548 (1993). https://doi.org/10.1007/BF02846755
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DOI: https://doi.org/10.1007/BF02846755