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Genetic analysis by chromosome-mediated gene transfer

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Summary

A general method is presented for stable transfer of genetic information to eukaryotic cells, utilizing metaphase chromosomes as the vehicle. Recent progress, current problems and large areas of uncertainty in this field are reviewed; particular consideration is given to frequency of transfer, size of the transgenome, evidence for cotransfer of linked genes and serial chromosome transfer. A reasonable model for chromosome transfer is considered with respect to the available information, and various discrepancies are noted. The utility of this method for fine structural mapping, cloning small regions of the eukaryotic genome and other potential applications are discussed.

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References

  1. McBride, O. W., and H. L. Ozer. 1973. Transfer of Genetic Information by Purified Metaphase Chromosomes. Proc. Natl. Acad. Sci. U.S.A. 70: 1258–1262.

    Article  PubMed  CAS  Google Scholar 

  2. McBride, O. W., and H. L. Ozer. 1973. Gene Transfer with Purified Mammalian Chromosomes. In: L. G. Silvestri (Ed.),Possible Episomes in Eukaryotes: Le Petit Colloquia on Biology and Medicine. Vol. 4. North-Holland, Amsterdam, pp. 255–267.

    Google Scholar 

  3. Shani, M., E. Huberman, Y. Aloni, and L. Sachs. 1974. Activation of Simian Virus 40 by Transfer of Isolated Chromosomes from Transformed Cells. Virology 61: 303–305.

    Article  PubMed  CAS  Google Scholar 

  4. Burch, J. W., and O. W. McBride. 1975. Human Gene Expression in Rodent Cells after Uptake of Isolated Metaphase Chromosomes. Proc. Natl. Acad. Sci. U.S.A. 72: 1797–1801.

    Article  PubMed  CAS  Google Scholar 

  5. Wullems, G. J., J. van der Horst, and D. Bootsma. 1975. Incorporation of Isolated Chromosomes and Induction of Hypoxanthine Phosphoribosyl-Transferase in Chinese hamster Cells. Som. Cell Genet. 1: 137–152.

    Article  CAS  Google Scholar 

  6. Willecke, K., and F. H. Ruddle. 1975. Transfer of the Human Gene for Hyposanthine-Guanine Phosphoribosyltransferase via Isolated Human Metaphase Chromosomes into Mouse L-Cells. Proc. Natl. Acad. Sci. U.S.A. 72: 1792–1796.

    Article  PubMed  CAS  Google Scholar 

  7. Ruddle, F. H., and O. W. McBride. 1976. New Approaches to Cell Genetics: Cotransfer of Linked Genetic Markers by Chromosome Mediated Gene Transfer. In: T’so P. (Ed.),The Molecular Biology of the Mammalian Genetic Apparatus: Its Relationship to Cancer, Aging, and Medical Genetics, Assoc. Scientific Pub., in press.

  8. Willecke, K., R. Lange, A. Kruger, and T. Reber. 1976. Cotransfer of two Linked Human Genes into Cultured Mouse Cells. Proc. Natl. Acad. Sci. U.S.A. 73: 1274–1278.

    Article  PubMed  CAS  Google Scholar 

  9. Athwal, R. S., and O. W. McBride. 1976. Sequential Transfer of a Human Gene through Mouse Cells to Hamster Cells by Incorporation of Isolated Metaphase Chromosomes. Fed. Proc. 35: 1661.

    Google Scholar 

  10. Degnen, G. E., I. L. Miller, J. M. Eisenstadt, and E. A. Adelberg. 1976. Chromosome-mediated Gene Transfer between Closely Related Strains of Cultured Mouse Cells. Proc. Natl. Acad. Sci. U.S.A. 73, in press.

  11. Notani, N. K., and J. K. Setlow. 1974. Mechanism of Bacterial Transformation and Transfection. In: W. Cohn (Ed.),Progress in Nucleic Acid Research and Molecular Biology. Vol. 14. Academic Press, New York and London, pp. 39–100.

    Google Scholar 

  12. Szybalska, E. H., and W. Szybalski. 1962. Genetics of Human Cell Lines, IV. DNA-Mediated Heritable Transformation of a Biochemical Trait. Proc. Natl. Acad. Sci. U.S.A. 48: 2026–2034.

    Article  PubMed  CAS  Google Scholar 

  13. Abrahams, P. J., and A. J. van der EB. 1975. In Vitro Transformation of Rat and Mouse Cells by DNA from Simian Virus 40. J. Virol. 16: 206–209.

    PubMed  CAS  Google Scholar 

  14. Mendelsohn, J., D. E. Moore, and N. P. Salzman. 1968. Separation of Isolated Chinese hamster Metaphase Chromosomes into Three Size-Groups. J. Mol. Biol. 32: 101–112.

    Article  PubMed  CAS  Google Scholar 

  15. Hearst, J. E., and M. Botchan. 1970. The Eukaryotic Chromosome. Annu. Rev. Biochem. 39: 151–182.

    Article  PubMed  CAS  Google Scholar 

  16. Maio, J. J., and C. L. Schildkraut. 1969. Isolated Mammalian Metaphase Chromosomes II. Fractionated Chromosomes of Mouse and Chinese hamster Cells. J. Mol. Biol. 40: 203–216.

    Article  PubMed  CAS  Google Scholar 

  17. Wray, W. 1973. Isolation of Metaphase Chromosomes with High Molecular Weight DNA at pH 10.5. In: D. M. Prescott (Ed.),Methods in Cell Biology. Vol. 6. Academic Press, New York and London, pp. 283–315.

    Google Scholar 

  18. McBride, O. W. 1974. Metaphase Chromosome Uptake by Mammalian Cells and Expression of the Genes Transferred. In: G. P. Redei, and G. Kimber (Eds.),Stadler Genetics Symposia. Vol. 6. University of Missouri, Columbia, Mo., pp. 53–75.

  19. Ittensohn, O. L., and D. J. Hutchison. 1969. Cytologic Manifestations of the Phagocytosis of L1210 Chromosomes by L1210 Cells in Culture. Exp. Cell Res. 55: 149–154.

    Article  PubMed  CAS  Google Scholar 

  20. Kato, H., K. Sekiya, and T. H. Yosida. 1971. Uptake of Isolated Chromosomes by Mammalian and Protective Effect of Protamine Sulfate. Exp. Cell Res. 65: 454–462.

    Article  PubMed  CAS  Google Scholar 

  21. Ebina, T., R. Miao, and Y. Watanabe. 1974. Transfer of Chromosomal Material. Association of Rescuable Sarcoma Virus Genome with the Chromosomal Fraction. Exp. Cell Res. 88: 203–206.

    Article  PubMed  CAS  Google Scholar 

  22. Tooze, J. 1973.The Molecular Biology of Tumor Viruses. Cold Spring Harbor Laboratory, pp. 329–331.

  23. Buck, D. W., S. J. Goss, and W. F. Bodmer. 1976. Regional mapping of the X-linked gene for a human cell surface antigen, SA-X. In:Birth Defects: Original Article Series. Third International Workshop on Human Gene Mapping, 1975. National Foundation, New York, in press.

    Google Scholar 

  24. Paris Conference (1971): Standardization in Human Cytogenetics. 1972. In: D. Bergsma (Ed.),Birth Defects: Original Article Series. Vol. 8, No. 7. National Foundation, New York, p. 4.

  25. Elsevier, S., R. S. Kucherlapati, E. A. Nichols, R. P. Creagan, R. E. Giles, F. H. Ruddle, K. Willecke and J. McDougall. 1974. Assignment of the gene for galacto-kinase to human chromosome 17 and its regional localisation to band q21–22. Nature 251: 633–636.

    Article  PubMed  CAS  Google Scholar 

  26. Nichols, E. A., S. M. Elsevier, and F. H. Ruddle. 1974. A New Electrophoretic Technique for Mouse, Human, and Chinese hamster Galactokinase. Cytogenet. Cell Genet. 13: 275–278.

    PubMed  CAS  Google Scholar 

  27. Deaven, L. L., and D. F. Petersen. 1974. Measurements of Mammalian Cellular DNA and Its Localization in Chromosomes. In: D. M. Prescott (Ed.),Methods in Cell Biology. Vol. 8. Academic Press, New York and London, p. 179–204.

    Google Scholar 

  28. Kunkel, L. M., K. D. Smith, and S. H. Boyers. 1976. Human Y-Chromosome-Specific Reiterated DNA. Science 191: 1189–1190.

    Article  PubMed  CAS  Google Scholar 

  29. Yosida, T. H., and T. Sekiguchi. 1968. Metaphase Figures of Rat Chromosomes Incorporated into Mouse Cells. Mol. Gen. Genetics 103: 253.

    Article  CAS  Google Scholar 

  30. Ebina, T., I. Kamo, K. Takahashi, M. Homma, and N. Ishida. 1970. Incorporation of Isolated HeLa-S3 Metaphase Chromosomes Into Cultured Mouse Embryo Cells. Exp. Cell Res. 62: 384–388.

    Article  PubMed  CAS  Google Scholar 

  31. Sekiguchi, T., F. Sekiguchi, and M. Yamada. 1973. Incorporation and Replication of Foreign Metaphase Chromosomes in Cultured Mammalian Cells. Exp. Cell Res. 80: 223–236.

    Article  PubMed  CAS  Google Scholar 

  32. Rao, P. N., and R. T. Johnson. 1972. Cell Fusion and Its Application to Studies on the Regulation of the Cell Cycle. In: D. M. Prescott (Ed.),Methods in Cell Physiology. Vol. 5. Academic Press, New York and London, pp. 75–126.

    Google Scholar 

  33. Schwartz, A. G., P. R. Cook, and H. Harris. 1971. Correction of a Genetic Defect in a Mammalian Cell. Nature 230: 5–8.

    CAS  Google Scholar 

  34. Boyd, Y. L., and H. Harris. 1973. Correction of Genetic Defects in Mammalian Cells by the Input of Small Amounts of Foreign Genetic Material. J. Cell Sci. 13: 841–861.

    PubMed  CAS  Google Scholar 

  35. Klinger, H. L., and S. Shin. 1974. Modulation of the Activity of an Avian Gene Transferred into a Mammalian Cell by Cell Fusion. Proc. Natl. Acad. Sci. U.S.A. 71: 1398–1402.

    Article  PubMed  CAS  Google Scholar 

  36. Ringertz, N. R., T. Ege, P. Elias, and E. Sidebottom. 1975. Reconstruction of Cells from Cell Fragments. In:Organization and Expression of the Eukaryotic Genome: Biochemical Mechanisms of Differentiation in Prokaryotes and Eukaryotes. Vol. 38. North-Holland Press, Amsterdam, pp. 235–244.

    Google Scholar 

  37. Fox, A. S., and S. B. Yoon. 1970. DNA-Induced Transformation in Drosophila; Locus-Specificity and the Establishment of Transformed Stocks. Proc. Natl. Acad. Sci. U.S.A. 67: 1608–1615.

    Article  PubMed  CAS  Google Scholar 

  38. Ledoux, L., and R. Huart. 1974. DNA-mediated genetic correction of thiamineless Arabidopsis thaliana. Nature 249: 17–21.

    Article  PubMed  CAS  Google Scholar 

  39. Degnen, G. E., I. L. Miller, E. A. Adelberg, and J. M. Eisenstadt. 1976. Expression of the gene for hypoxanthine-guanine phosphoribosyl transferase after transfer between mouse cells. Fed. Proc. 35: 1661.

    Google Scholar 

  40. Wullems, G. J., J. van der Horst, and D. Bootsma. 1976. Expression of Human Hyposanthine Phosphotibosyl Transferase in Chinese hamster Cells Treated with Isolated Human Chromosomes. Som. Cell Genet. 2: 155–164.

    Article  CAS  Google Scholar 

  41. Wullems, G. J., J. van der Horst, and D. Bootsma. 1976. Transfer of the Human X Chromosome to Human-Chinese Hamster Cell Hybrids via Isolated HeLa Metaphase Chromosomes. Som. Cell Genet. 2: in press.

  42. Gray, J. W., A. V. Carrano, L. L. Steinmetz, M. A. Van Dilla, D. H. Moore II, B. H. Mayall, and M. L. Mendelsohn. 1975. Chromosome Measurement and Sorting by Flow Systems. Proc. Natl. Acad. Sci. U.S.A. 72: 1231–1234.

    Article  PubMed  CAS  Google Scholar 

  43. Stubblefield, E., S. Cram, and L. Deaven. 1975. Flow microfluorometic analysis of isolated Chinese hamster chromosomes. Exp. Cell Res. 94: 464–468.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Biochemistry, National Cancer Institute, NIH, 20014, Bethesda, MA

    O. W. McBride M.D. & R. S. Athwal

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  1. O. W. McBride M.D.
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  2. R. S. Athwal
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McBride, O.W., Athwal, R.S. Genetic analysis by chromosome-mediated gene transfer. In Vitro 12, 777–786 (1976). https://doi.org/10.1007/BF02835452

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