Conclusions
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1)
P32 loaded on the duodenum was excreated into bile after 10–15 minutes (The peak; 6–7 hours after injection). The excreation was continued still after 24 hours.
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2)
P32 loaded on the duodenum was excreated into bile 1.7% after 8 hours and 4.7% after 24 hours.
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3)
The radioactivity of P32 on paperchromatogram was detected on the spot corresponding to ester-form bilirubin and could not be observed in other bilirubin fractions.
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4)
Only the ester-form bilirubin fraction, separated by Kosaka-Hara’s method showed P32-radioactivity.
These results suggest that bilirubin phosphate is one part of ester-form bilirubin.
References
Kosaka, K. Iwahara, M. & Kondo, T.: Proc. VIII, Invest. Congr. Haemat, 2, 1247 Tokyo, 1962.
Kondo, T.: 10th Anniversary Symposium on Glucuronic Acid Report 63–70, 1964.
Kawaharada, H.: Igaku-Kenkyu (Acta Medica) 37, 54–62, 1967.
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Kondo, T., Kawai, T. & Takagi, M. Studies on bilirubin phosphate by P32 . Gastroenterol Jpn 3, 116 (1968). https://doi.org/10.1007/BF02827157
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DOI: https://doi.org/10.1007/BF02827157