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Integrated bombardment andAgrobacterium transformation system: An alternative method for soybean transformation

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Abstract

Described here is a new method for soybean transformation, based on microwounding of embryonic clumps by particle bombardment prior to inoculation with anAgrobacterium suspension. The method combines the advantages of somatic embryogenesis and gene transfer through an integrated transformation system. This is the first report of application of this technique to transformation ofGlycine max.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

hpt :

gene for hygromicin phosphotransferase

gusA-intron:

gene for β-glucuronidase with an intron

OD:

optic density

Tween 20:

polyoxyethylenesorbitan

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Authors and Affiliations

  1. Centro de Ciências da Saúde, Universidade do Vale do Rio dos Sinos, Caixa Postal 275, 93022-000, São Leopoldo, RS, Brazil

    Annette Droste (Setor de Botânica)

  2. Centro de Biotecnologia do Estado do Rio Grande do Sul e Departamento de Biotecnologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Caixa Postal 15005, 91501-970, Porto Alegre, RS, Brazil

    Giancarlo Pasquali

  3. Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91501-970, Porto Alegre, RS, Brazil

    Maria Helena Bodanese-Zanettini

Authors
  1. Annette Droste
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  2. Giancarlo Pasquali
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  3. Maria Helena Bodanese-Zanettini
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Correspondence to Annette Droste.

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Droste, A., Pasquali, G. & Bodanese-Zanettini, M.H. Integrated bombardment andAgrobacterium transformation system: An alternative method for soybean transformation. Plant Mol Biol Rep 18, 51–59 (2000). https://doi.org/10.1007/BF02825294

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