Abstract
We have generated transgenic plants of a Brazilian elite Indica-type rice by electroporation of shoot apices. This approach avoids a callus phase and produces 0.4–13.8% resistant plants. Transgenic plantlets were transferred to soil a few weeks after explant electroporation. Root segments from plantlets obtained from transformation experiments with pAHC25 plasmid were GUS positive. Integration of the introduced gene into the genome was demonstrated by PPT and antibiotic screening as well as by PCR and Southern blot hybridization of genomic DNA isolated from R2 plantlets.
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Abbreviations
- ASPm:
-
ASP modified electroporation buffer
- G418:
-
geneticin
- Hyg:
-
hygromycin
- PPT:
-
phosphinotricin
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Muniz de Péadua, V.L., Ferreira, R.P., Meneses, L. et al. Transformation of Brazilian eliteIndica-type rice (Oryza sativa L.) by electroporation of shoot apex explants. Plant Mol Biol Rep 19, 55–64 (2001). https://doi.org/10.1007/BF02824078
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DOI: https://doi.org/10.1007/BF02824078