Summary
TheAntirrhinum majus Tam3 element was introduced intoArabidopsis thaliana protoplasts and plants in order to assess the influence of anin vitro culture phase such as protoplasts and callus culture on the mobility of this transposable element in this plant species. The constructs used contained theTam3 element inserted in between the CaMV 35S promoter and thegus- orhpt-coding region, allowing a direct selection of excision candidates. From the different approaches used, only a long-term callus culture allowed us to detectTam3 activity. NoTam3 activity could be detected in protoplasts or protoplast-derived microcolonies. Our data are compared with those previously reported forTam3 in tobacco and petunia.
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De Greef, B., Jacobs, M. Evidence forTAM3 activity in transgenicArabidopsis thaliana . In Vitro Cell.Dev.Biol.-Plant 32, 241–248 (1996). https://doi.org/10.1007/BF02822694
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DOI: https://doi.org/10.1007/BF02822694