Skip to main content
Log in

Isolation of wildXanthomonas strains from agricultural produce, their characterization and potential related to polysaccharide production

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

One hundred fifty wild strains ofXanthomonas were isolated on five selective and nonselective media. The comparative effectivity of the five media has been discussed. Fifteen polysaccharide-producing strains shortlisted were identified up to species level. These were studied for polysaccharide production in shake flasks using YM media. The highest final yield of 21.3 g/L of the amount of carbon source supplied has been obtained in the optimum medium in shake-flask experiments fromXanthomonas campestris ICa-125 strain isolated from cabbage. Rheological properties of the exocellular polysaccharide (EPS) have been compared with standard xanthan. EPS fromX. campestris ICa-125 was found to be superior with respect to heat stability and effect of electrolytes as compared to standard xanthan.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Albrecht W.J., Sohns U.E., Rogovin S.P.: Pilot-plant process for the isolation of a microbial polysaccharide with a quaternary ammonium compound.Biotechnol. Bioeng.5, 91–99 (1963).

    Article  CAS  Google Scholar 

  • Breed R.S.:Bergey's Manual of Determinative Bacteriology, 7th ed. (R.S. Breed, E.G.D. Murray, N.R. Smith, Eds), pp. 152–178. Williams & Wilkins, Baltimore 1957.

    Google Scholar 

  • Cadmus M.C., Rogovin S.P., Burton K.A., Pittsley J.E., Knutson C.A., Jeanes A.: Colonial variation inXanthomonas campestris NRRL B-1459 and characterization of the polysaccharide from a variant strain.Can. J. Microbiol.22, 942–948 (1976).

    PubMed  CAS  Google Scholar 

  • Cadmus M.C., Knutson C.A., Lagoda A.A., Pittsley J.E., Burton K.A.: Synthetic media for production of quality xanthan gum in 20 liter fermentors.Biotechnol. Bioeng.20, 1003–1014 (1978).

    Article  CAS  Google Scholar 

  • Chin-Hang Shu, Shang-Tian Yang: Kinetics and modelling of temperature effect on batch xanthan gum fermentation.Biotechnol. Bioeng.37, 567–574 (1991).

    Article  Google Scholar 

  • Fernando F., Torres L.G., Galindo E.: Effect of dissolved oxygen tension during cultivation ofX. campestris on the production and quality of xanthan gum.J. Biotechnol.34, 165–173 (1994).

    Article  Google Scholar 

  • Garcia-Ochoa F., Casas J.A., Mohedano A.F.: Xanthan precipitation from solutions and fermentation broths.Separat. Sci. Technol.28, 1303–1313 (1993).

    Article  Google Scholar 

  • Harper H.A.:Carbohydrates: Review of Physiological Chemistry, 14th ed. Large Medical Publication, Maruzen Asian Co., Los Altos (California) 1973.

    Google Scholar 

  • Hassler R.A., Doherty D.H.: Genetic engineering of polysaccharide structure: production of variants of xanthan gum inXanthomonas campestris.Biotechnol. Progr.6, 182–187 (1990).

    Article  CAS  Google Scholar 

  • Jeanes A., Rogovin P., Cadmus M.C., Silman R.W., Knutson C.A.:Polysaccharide (Xanthan) of Xanthomonas campestrisNRRL B-1459: Procedures for Culture Maintenance and Polysaccharide Production, Purification and Analysis, ARS-NC 51, Agricultural Research Service, USDA, Peoria (USA) 1976.

    Google Scholar 

  • Kado C.I., Heskett M.G.: Selective media for isolation ofAgrobacterium, Corynebacterium, Erwinia, Pseudomonas & Xanthomonas.Phytopathology60, 969–976 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Kennedy J.F., Jones P., Barker S.A.: Factors affecting microbial growth and polysaccharide production during the fermentation ofXanthomonas campestris culture.Enzyme Microbiol. Biotechnol.4, 39–43 (1982).

    Article  CAS  Google Scholar 

  • Kennedy J.F., Bradshaw I.J.: Production properties and applications of xanthan.Progr. Industr. Microbiol.19, 319–371 (1984).

    CAS  Google Scholar 

  • Koníček J., Lasík J., Wurst M.: Production and characteristics of the exocellular polysaccharide in mutant strains ofXanthomonas fuscans.Folia Microbiol.22, 12–18 (1977).

    Article  Google Scholar 

  • Luc De Vuyst, Jan Van Loo, Vandamme E.J.: Two step fermentation process for improved xanthan production byXanthomonas campestris NRRL B-1459.J. Chem. Technol. Biotechnol.39, 263–273 (1987).

    Google Scholar 

  • Luc De Vuyst, Vermeire A.: Use of industrial medium components for xanthan production byXanthomonas campestris NRRL B-1459.Appl. Microbiol. Biotechnol.42, 187–191 (1994).

    Google Scholar 

  • Martinez-Salazar J.M., Palacios A.M., Sanchez R., Caro A.D., Soberon-Chavez G.: Genetic stability and xanthan gum production inXanthomonas campestris pv.campestris NRRL B-1459.Mol. Microbiol.8, 1053–1061 (1993).

    Article  PubMed  CAS  Google Scholar 

  • Millane R.P., Narasaiah T.V.: X-ray fiber diffraction studies of a variant of xanthan gum in which the sidechain terminal mannose unit is absent.Carbohydr. Polym.12, 315–321 (1990).

    Article  CAS  Google Scholar 

  • Moraine R.A., Rogovin P.: Xanthan biopolymer production at increased concentration by pH control.Biotechnol. Bioeng.13, 381–391 (1971).

    Article  CAS  Google Scholar 

  • Moraine R.A., Rogovin P.: Kinetics of xanthan fermentation.Biotechnol. Bioeng.15, 225–237 (1973).

    Article  CAS  Google Scholar 

  • Nitschke M., Thomas R.W.S.P.: Xanthan gum production by wild-type isolates ofXanthomonas campestris.World J. Microbiol. Biotechnol.11, 502–504 (1995).

    Article  CAS  Google Scholar 

  • Ramirez M.E., Fucikovsky L., Garcia-Jimenez F., Quintero R., Galindo E.: Xanthan production by altered pathogenicity variants ofXanthomonas campestris.Appl. Microbiol. Biotechnol.29, 5–10 (1988).

    Article  CAS  Google Scholar 

  • Roseiro J.C., Costa D.C., Collaco M.T.A.: Batch and fed-batch cultivation ofXanthomonas campestris in carob extracts.Lebensm. Wiss. Technol.25, 289–293 (1992).

    CAS  Google Scholar 

  • Roseiro J.C., Girio F.M., Kara A., Collaco M.T.A.: Kinetic and metabolic effects of nitrogen, magnesium and sulphur restriction inXanthomonas campestris batch cultures.J. Appl. Bacteriol.75, 381–386 (1993).

    CAS  Google Scholar 

  • Schaad N.W., White W.C.: A selective medium for soil isolation and enumeration ofXanthomonas campestris.Phytopathology64, 876–880 (1974).

    Article  Google Scholar 

  • Sharma A., Nair P.M., Pawar S.E.: Identification of soybean strains resistant toXanthomonas campestris pv.glycines.Euphytica67, 95–99 (1993).

    Article  Google Scholar 

  • Starr M.P.: The genusXanthomonas, pp. 742–763 inThe Procaryotes, Vol. 1, 2nd ed. (M.P. Starr, H. Stolp, H.G. Truper, A. Balows, H.G. Schlegel, Eds). Springer Verlag, Berlin-Heidelberg 1986.

    Google Scholar 

  • Tait M.I., Sutherland I.W., Clarke-Sturman A.J.: Effect of growth conditions on the production, composition and viscosity ofXanthomonas campestris exopolysaccharide.J. Gen. Microbiol.132, 1483–1492 (1986).

    CAS  Google Scholar 

  • Trilsbach J.F., Pielken P., Hamacher K., Sahm H.: Xanthan formation byXanthomonas campestris under different culture conditions, pp. 65–70 inThird European Congress on Biotechnology, Vol. II. Verlag Chemie, Berlin 1984.

    Google Scholar 

  • Vermani M.V., Kelkar S.M., Kamat M.Y.: Novel polysaccharide produced byAzotobacter vinelandii isolated from plant rhizosphere.Biotechnol. Lett.17, 917–920 (1995).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gupte, M.D., Kamat, M.Y. Isolation of wildXanthomonas strains from agricultural produce, their characterization and potential related to polysaccharide production. Folia Microbiol 42, 621–628 (1997). https://doi.org/10.1007/BF02815476

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02815476

Keywords

Navigation