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The isolation and purification of pre-S2 containing hepatitis B virus surface antigen by chemical affinity chromatography

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Abstract

A simple, rapid, and efficient method was developed to isolate and purify pre-S2 containing HBsAgs from the plasma of a single chronic carrier of HBsAg (adw) by ammonium sulfate fractionation, hydroxyapatite column chromatography, and polymerized human serum albumin-affinity column chromatography. About 500 µg of pre-S2 containing HBsAg was obtained from 140 mL of plasma containing 4,200 µg of HBsAg. Two purified pre-S2 containing HBsAgs were analyzed by SDS-polyacrylamide gel electrophoresis and their molecular weights were determined to be 31,000 and 68,000 respectively. No significant amount of HBsAg or its derivative was detected in the final product.

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Authors and Affiliations

  1. Institute of Biochemistry, College of Medicine, National Taiwan University, 10018, Taipei, Taiwan, R.O.C.

    Jung-Yaw Lin, Yih-Shou Hsieh & Shu-Chen Chu

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  1. Jung-Yaw Lin
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  2. Yih-Shou Hsieh
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  3. Shu-Chen Chu
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Lin, JY., Hsieh, YS. & Chu, SC. The isolation and purification of pre-S2 containing hepatitis B virus surface antigen by chemical affinity chromatography. Appl Biochem Biotechnol 15, 255–263 (1987). https://doi.org/10.1007/BF02798453

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  • DOI: https://doi.org/10.1007/BF02798453

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