Summary
A rapid procedure for the preparation of delipidized serum protein is described. The delipidized protein can be used for the maintenance and growth of tissue culture cells in a lipid-free environment. The extraction procedure greatly reduces all serum lipid classes and the delipidized protein supports the growth of a variety of cells in culture.
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This research was supported in part by USPHS research grants HL-09103 and HL-16058 from the National Heart and Lung Institute and RR-05540 from the Division of Research Resources. This work was done during tenure as Established Investigator of the American Heart Association (G.H.R.).
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Rothblat, G.H., Arbogast, L.Y., Ouellette, L. et al. Preparation of delipidized serum protein for use in cell culture systems. In Vitro Cell.Dev.Biol.-Plant 12, 554–557 (1976). https://doi.org/10.1007/BF02797438
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DOI: https://doi.org/10.1007/BF02797438