Abstract
The nuclear morphology of WI-38 cells plated on increasing thicknesses of the polymer, poly-2-hydroxyethylmethacrylate, was studied. Changes in nuclear morphometry were found to parallel changes in cellular morphometry: namely a transition to a more rounded and compact conformation, with increasing thicknesses of the polymer. Such changes in nuclear morphometry were opposite to those already observed after stimulation of proliferation by serum addition and similar to those observed with increasing confluency. These results suggest that coupling between cell and nuclear geometry may represent a mechanism for the control of proliferation by cell shape in nontransformed cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Folkman, J., and Greenspan, H. P. (1975),Biochim. Biophys. Acta 417, 211.
Folkman, J., and Moscona, A. (1978),Nature 273, 345.
Adam, G., Ernst, M., and Seher, J. P. (1979),Exptl. Cell Res. 120, 127.
Nicolini, C., Giaretti, W., DeSaive C., and Kendall, F. (1977),Exptl. Cell Res. 106, 119.
Kendall, F., Wu, S., Giaretti, W., and Nicolini, C. (1977),J. Histochem. Cytochem. 25, 724.
Nicolini, C., Linden, W. A., Zietz, S., and Wu, C. T. (1977),Nature 270, 607.
Nicolini, C., Kendall, F., and Giaretti, W. (1977),Biophys. J. 19, 163.
Kendall, F., Swenson, R., Borun, T., Rowinski, J., and Nicolini, C. (1977),Science 196, 1106.
Nicolini, C., and Beltrame, F. (1980),Proc. Natl. Acad. Sci. USA, in press.
Kendall, F., Beltrame, F., and Nicolini, C. (1979),IEEE Trans. Biomed. Eng. 3, 172.
Nicolini, C. (1979), inChromatin Structure and Function, Part B, (Nicolini, C., ed.), Plenum, New York, pp. 613–667.
Kendall, F., Beltrame, F., and Nicolini, C. (1979),Chromatin Structure and Function, Part A (Nicolini, C., ed.), Plenum, New York, pp. 265–292.
Sawicki, W. (1979) inChromatin Structure and Function, Part B. (Nicolini, C., ed.), Plenum, New York, pp. 667–679.
Hsie, A., and Puck, J. (1971),Proc. Natl. Acad. Sci. USA 68, 358.
Puck, J., Waldren, C. A., and Hsie, A. (1972),Proc. Natl. Acad. Sci. USA 69, 1943.
Crusberg, T. C., Hoskins, B. B., and Widdas, R. (1979),Exptl. Cell Res. 118, 39.
Teng, M., Bartholomew, J. C., and Bissell, M. (1977),Nature 268, 739.
Mazia, D., Schatten, G., and Sale, W. (1975),J. Cell Biol. 66, 198.
Nicolini, C., (1980),J. Submicro. Cytol. 12, 3.
Dulbecco, R., and Elkington, J. (1975),Proc. Natl. Acad. Sci. USA 72, 1584.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Belmont, A.S., Kendall, F.M. & Nicolini, C.A. Coupling of nuclear morphometry to cell geometry and growth in human fibroblasts. Cell Biophysics 2, 165–175 (1980). https://doi.org/10.1007/BF02795842
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02795842