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Inflammation of the liver causes mutations in duck hepatitis B virus genome

  • Liver, Pancreas, and Biliary Tract
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Summary

To investigate whether hepatitis causes mutation in the viral genome, DNA sequences in the pre-core region of duck hepatitis B virus (DHBV) DNA were analyzed in both ducks with hepatitis and without hepatitis. Five DHBV carrier ducks were injected with DHBV particle proteins purified from duck serum with Freund’s complete adjuvant (FCA) intrahepatically from 14 day posthatch for 9 weeks (immunized group). Serum was drawn at the end of the 1st and 4th week after the 1st injection of DHBV particle protein and ducks were killed at the end of the 9th week to obtain the liver. Another five ducks without treatment were used as controls. All ducks of the immunized group showed moderate to severe hepatitis at the 9th week. All ducks in the immunized group showed one mutation except one duck that showed two mutations only at the 9th week. Mutations were observed in the 5th, 13th, 21st, 22nd, and 28th codon of the precore region. All of them were point mutation at the 3rd base in the triplets. The frequency of mutation was different in each duck from 20% to 60% but not 100%. There was no mutations in ducks in control group. These results suggest that hepatitis causes mutation in the pre-core lesion genome of duck hepatitis B virus.

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References

  1. Carman WF, Jacyna MR, Hadziyannis S, et al. mutation preventing formation of hepatitis B e antigen in patients with chronic hepatitis B infection. Lancet ii;1989:588–591.

    Article  Google Scholar 

  2. Kosaka Y, Takase K, Kojima M, et al. fulmint hepatitis B: Induction by hepatitis B virus mutants defective in the precore region and incapable of encoding e antigen. Gastroenterology 1991;100: 1087–1094.

    PubMed  CAS  Google Scholar 

  3. Omata M, Ehata T, Yokosuka O, et al. Mutation in the precore region of hepatitis B virus in patients with fluminant hepatitis. N Engl J Med 1991;324:16991704.

    Article  Google Scholar 

  4. Liang TJ, Hasegawa K, Rimon N, et al. A hepatitis B virus mutant associated with an epidemic of fluminant hepatitis. N Engl J Med 1991;324:1705–1709.

    Article  PubMed  CAS  Google Scholar 

  5. Terazawa S, Kojima M, Yamanaka T, et al. Hepatitis B virus mutants with precore-region defects in two babies with fluminant hepatitis and their mothers positive for antibody to hepatitis B virus e antigen. Pediatr Res 1991;29:5–9.

    Article  PubMed  CAS  Google Scholar 

  6. Okamoto H, Yotsumoto S, Akahane Y, et al. Hepatitis B virus with precore region defects prevail in persistently infected host along with seroconversion to the antibody against e antigen. J Virol 1990;64:1298–1303.

    PubMed  CAS  Google Scholar 

  7. Takeda K, Akahane Y, Suzuki H, et al. Defects in the precore region of the HBV genome in patients with chronic hepatitis B after sustained seroconversion from HBeAg to anti-HBe induced spontaneously or with interferon therapy. Hepatology 1990;12: 1284–1289.

    Article  PubMed  CAS  Google Scholar 

  8. Carman WF, Zanetti AR, Karayiannis P, et al. Vaccine-induced escape mutant of hepatitis B virus. Lancet 1990;336:325–329.

    Article  PubMed  CAS  Google Scholar 

  9. Mason WS, Seal G, Summers J. Virus of pekin ducks with structural and biological relatedness to human hepatitis B virus. J Virol 1980;36:829–836.

    PubMed  CAS  Google Scholar 

  10. Fukuda R, Fukumoto S, Shimada Y. A sequential study of viral DNA in serum in experimental transmission of Duck hepatitis B virus. J Med Virol 1987;21:311–320.

    Article  PubMed  CAS  Google Scholar 

  11. Akagi S, Fukuda R, Shimada Y. Sequential changes of serum 2′,5′-oligoadenylate synthetase in experimental transmission of duck hepatitis B virus. Gastroenterol Jpn 1992;27:374–381.

    PubMed  CAS  Google Scholar 

  12. Summers J, Mason WS. Replication of the genome of a hepatitis B virus-like virus by revers transcription of an RNA intermediate. Cell 1982;29:403–415.

    Article  PubMed  CAS  Google Scholar 

  13. Kohge N, Fukuda R. Attempt to cause hepatitis in duck hepatitis B virus carrier ducks by immunization with DHBV protein. Jpn J Gastroenterol 1992;89:1242–1251 (in Japanese)

    CAS  Google Scholar 

  14. Uchida M, Esumi M, Shikata T. Molecular cloning and sequence analysis of duck hepatitis B virus genom of a new variant isolated from shanghai ducks. J Med Virol 1989;173:600–606.

    CAS  Google Scholar 

  15. Marion PL, Knight SS, Fettelson MA, et al. Major polypeptide of duck hepatitis B virus surface antigen particles. J Virol 1983;48: 534–541.

    PubMed  CAS  Google Scholar 

  16. Marion PL, Kight SS, Ho BK, et al. Liver disease aswsociated with duck hepatitis B virus infection of domestic ducks. Proc Natl Acad Sci USA 1984;81:898–902.

    Article  PubMed  CAS  Google Scholar 

  17. Fukuda R, Okinaga S, Akagi S, et al. Alteration of infection pattern of duck hepatitis B virus by immunomodulatory drugs. J Med Virol 1988;26:387–396.

    Article  PubMed  CAS  Google Scholar 

  18. Bruss V, Gerlich WH. Formation of transmembranous Hepatitis B e-antigen by cotranslational in vitro processing of the viral precore protein. Virology 1988;163:268–275.

    Article  PubMed  CAS  Google Scholar 

  19. Garcia PD, Ou JH, Rutter WJ. Targeting of the hepatitis B virus precore protein to the endoplasmic reticulum membrane: after signal peptide clevage translocation can be aborted and the product released into the cytoplasm. J Cell Biol 1988;106:1093–1104.

    Article  PubMed  CAS  Google Scholar 

  20. Shhlicht HG, Salfeld J, Schaller H. The duck hepatitis B viru pre-C region encodes a signal sequence which is essential for synthesis and secretion of processed core protein but not virus formation. J Virol 1987;61:3701–3709.

    Google Scholar 

  21. Shafritz D, Shouval D, Sherman H, et al. Integration of hepatitis V virus DNA in to the genome of liver cells in chronic liver disease and hepatocellular carcinoma. N Engl J Med 1981;305:1067–1073.

    Article  PubMed  CAS  Google Scholar 

  22. Brechot C, Hadchouel M, Scott J, et al. State of hepatitis B virus DNA in liver disease. Hepatology 1982;2:27S-34S.

    Google Scholar 

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Authors and Affiliations

  1. Second Department of Internal Medicine, Shimane Medical University, 89-1 Enya-Cho, IzumoShi, 693, Shimane, Japan

    Ryo Fukuda M.D., Naruaki Kohge, Shuji Akagi, Nguten Thanh Xuan, Alejandro Tokuda & Shiro Fukumoto

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  1. Ryo Fukuda M.D.
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  2. Naruaki Kohge
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  3. Shuji Akagi
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  4. Nguten Thanh Xuan
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  5. Alejandro Tokuda
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  6. Shiro Fukumoto
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Fukuda, R., Kohge, N., Akagi, S. et al. Inflammation of the liver causes mutations in duck hepatitis B virus genome. Gastroenterol Jpn 28, 254–258 (1993). https://doi.org/10.1007/BF02779228

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  • DOI: https://doi.org/10.1007/BF02779228

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