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Regulation of14C-cholesterol uptake from mixed micelles by organ culture of the mucosa epithelium from human small intestine: Effects of selective ACAT inhibitor

  • Biophysics and Biochemistry
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The initial rate of14C-cholesterol uptake from mixed micelles is studied using organ culture of mucosal epithelium from human small intestine obtained during surgery. Intense, reproducible, and ACAT-regulated level of specific14C-cholesterol uptake is monitored during the first 3 h of culturing. The specific14C-cholesterol uptake is assessed by the difference between the total uptake of14C-cholesterol and nonspecific uptake of14C-sitosterol from the micelles. It is shown that less than 20% of absorbed14C-cholesterol is converted into cholesterol esters. C44788, a high-affinity and selective ACAT inhibitor, inhibits14C-cholesterol uptake in a dose-dependent manner and practically has no effect on14C-oleate uptake.

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Authors and Affiliations

  1. Laboratory of Cell and Tissue Cultures, Institute of Experimental Cardiology, Cardiology Research Center, Russian Academy of Medical Sciences, USSR

    V. S. Repin, A. V. Bocharov, T. G. Vishnyakova, I. N. Baranova, L. Kornelius, M. A. Kornyushin, A. G. Talalaev, L. N. Kask, O. V. Mironyuk, A. V. Pokrovskii & A. E. Zotikov

  2. 1st Municipal Children Hospital, USSR

    M. A. Kornyushin, A. G. Talalaev, L. N. Kask & O. V. Mironyuk

  3. A. V. Vishnevskii Surgery Institute, Russian Academy of Medical Sciences, Moscow

    A. V. Pokrovskii & A. E. Zotikov

Authors
  1. V. S. Repin
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  2. A. V. Bocharov
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  3. T. G. Vishnyakova
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  4. I. N. Baranova
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  5. L. Kornelius
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  6. M. A. Kornyushin
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  7. A. G. Talalaev
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  8. L. N. Kask
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  9. O. V. Mironyuk
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  10. A. V. Pokrovskii
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  11. A. E. Zotikov
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Additional information

Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 123, No. 2, pp. 165–170, February, 1997

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Repin, V.S., Bocharov, A.V., Vishnyakova, T.G. et al. Regulation of14C-cholesterol uptake from mixed micelles by organ culture of the mucosa epithelium from human small intestine: Effects of selective ACAT inhibitor. Bull Exp Biol Med 123, 139–143 (1997). https://doi.org/10.1007/BF02766435

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  • DOI: https://doi.org/10.1007/BF02766435

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