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Proteolytic specificity of plasmin toward adhesive proteins

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Abstract

We showed that plasmin specifically hydrolyzes proteins participating in cell adhesion (fibronectin, vitronectin, and laminin) and does not affect nonadhesive proteins, such as albumin, ovalbumin, and alkaline phosphatase. These and some earlier results allow us to speak of the specific antiadhesive activity of plasmin, which significantly extends the concepts regarding the functional importance of this protein and indicates that it directly participates in the regulation of cell adhesion, the remodeling of the extracellular matrix, and, therefore, in cell migration.

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References

  1. Ingber, D.E.,J. Cell Sci., 1993, vol. 104, pp. 613–627.

    PubMed  Google Scholar 

  2. Juliano, R.L. and Haskill, S.,J. Cell Biol., 1993, vol. 120, pp. 577–585.

    Article  PubMed  CAS  Google Scholar 

  3. Sims, J.R., Karp, S., and Ingber, D.E.,J. Cell Sci., 1992, vol. 103, pp. 1215–1222.

    PubMed  Google Scholar 

  4. Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., and Watson, J.D.,Molecular Biology of the Cell, New York: Garland, 1983. Translated under the titleMolekulyarnaya biologiya kletki, Moscow: Mir, 1994, vol. 2, pp. 522–529.

    Google Scholar 

  5. Mignatti, P. and Rifkin, D.B.,Physiol. Rev., 1993, vol. 73, pp. 161–195.

    PubMed  CAS  Google Scholar 

  6. Moscatelli, D. and Rifkin, D.B.,Biochim. Biophys. Acta, 1988, vol. 948, pp. 67–85.

    PubMed  CAS  Google Scholar 

  7. Eaton, D.L., Scott, R.W., and Baker, J.B.,J. Biol. Chem., 1984, vol. 259, pp. 6241–6247.

    PubMed  CAS  Google Scholar 

  8. Matrisian, L.M.,BioEssays, 1992, vol. 14, pp. 455–463.

    Article  PubMed  CAS  Google Scholar 

  9. Werb, Z.,Cell, 1997, vol. 91, pp. 439–442.

    Article  PubMed  CAS  Google Scholar 

  10. Wong, A.P., Contez, Sh.L., and Baricos, W.H.,Am. J. Physiol., 1992, vol. 263, pp. F1112-F1118.

    PubMed  CAS  Google Scholar 

  11. Muranova, T., Shvyrkova, I., Arkhipov, V., and Rykunova, A.,Fibrinolysis Proteolysis, 1998, vol. 12, pp. 23–32.

    Article  CAS  Google Scholar 

  12. Muranova, T.A., Shvyrkova, I.G., and Rykunova, A.I.,Bioorg. Khim., 1999, vol. 26, pp. 187–191.

    Google Scholar 

  13. Deutch, D.G. and Mertz, E.T.,Science, 1970, vol. 170, pp. 1095–1096.

    Article  Google Scholar 

  14. Alkjaersig, N., Fletcher, A.P., and Sherry, S.,J. Biol. Chem., 1958, vol. 233, pp. 81–85.

    PubMed  CAS  Google Scholar 

  15. Dufour, S., Duband, J.-L., and Thiery, J.-P.,Biol. Cell, 1986, vol. 58, pp. 1–14.

    PubMed  CAS  Google Scholar 

  16. Regnault, V., Rivat, C., and Stolz, J.F.,J. Chromatogr., 1988, vol. 432, pp. 93–102.

    Article  PubMed  CAS  Google Scholar 

  17. Skorstengaard, K., Thogersen, H.C., Vibe-Pedersen, K., Petersen, T.E., and Magnusson, S.,Eur. J. Biochem., 1982, vol. 128, pp. 605–623.

    Article  PubMed  CAS  Google Scholar 

  18. McDonagh, R.P., McDonagh, J., Petersen, T.E., Thogersen, H.C., Skorstengaard, K., Sottrup-Jensen, L., and Magnusson, S.,FEBS Lett., 1981, vol. 127, pp. 174–178.

    Article  PubMed  CAS  Google Scholar 

  19. Thogersen, H.C., Petersen, T.E., Skorstengaard, K., Vibe-Pedersen, K., McDonagh, R., McDonagh, J., Magnusson, S., and Sottrup-Jensen, L., Abstracts of Papers,Protides Biological Fluids, Brussels, 1980, Abstr. no. 28.

  20. Gold, L.I., Schwimmer, R., and Quigley, J.P.,Biochem. J., 1989, vol. 262, pp. 529–534.

    PubMed  CAS  Google Scholar 

  21. Yatohgo, T., Izumi, M., Kashiwagi, H., and Hayashi, M.,Cell Struct. Funct., 1988, vol. 13, pp. 281–292.

    Article  PubMed  CAS  Google Scholar 

  22. Kost, Ch., Benner, K., Stockmann, A., Linder, D., and Preissner, K.T.,Eur. J. Biochem., 1996, vol. 236, pp. 682–688.

    Article  PubMed  CAS  Google Scholar 

  23. Luckenbill-Edds, L.,Brain Res. Rev., 1997, vol. 23, pp. 1–27.

    Article  PubMed  CAS  Google Scholar 

  24. Preissner, K.T.,Biophys. Biochem. Res. Commun., 1990, vol. 168, pp. 966–971.

    Article  CAS  Google Scholar 

  25. Knudsen, B.S., Silverstein, R.L., Leung, L.L.K., Harpel, P.C., and Nachman, R.L.,J. Biol. Chem., 1986, vol. 261, pp. 10765–10771.

    PubMed  CAS  Google Scholar 

  26. Silverstein, R.L., Leung, L.L.K., Harpel, P.C., and Nachman, R.L.,J. Clin. Invest., 1985, vol. 75, pp. 2065–2073.

    Article  PubMed  CAS  Google Scholar 

  27. Salonen, E.M., Saksela, O., Vartio, T., Nielson, L.S., and Zeuthen, J.,J. Biol. Chem., 1985, vol. 260, pp. 12 302–12 307.

    CAS  Google Scholar 

  28. Lawler, J.W. and Slayter, H.S.,Thrombosis Research, 1981, vol. 22, pp. 267–279.

    Article  PubMed  CAS  Google Scholar 

  29. Hamilton, K.K., Fretto, L.J., Griersa, D.S., and McKee, P.A.,J. Clin. Invest., 1985, vol. 76, pp. 261–270.

    PubMed  CAS  Google Scholar 

  30. Liotta, L.A., Goldfarb, R.H., Brundage, R., Siegal, G.P., Terranova, V., and Garbisa, S.,Cancer Res., 1981, vol. 41, pp. 4629–4636.

    PubMed  CAS  Google Scholar 

  31. Sane, D.C., Moser, T.L., and Greenberg, C.S.,Thrombosis Haemostasis, 1991, vol. 66, pp. 310–314.

    CAS  Google Scholar 

  32. Chain, D., Kreizman, T., Shapira, H., and Shaltid, S.,FEBS Lett., 1991, vol. 285, pp. 251–256.

    Article  PubMed  CAS  Google Scholar 

  33. Affinity Chromatography: Principles and Methods, Pharmacia Fine Chemicals, 1979, pp. 10–15.

  34. Walther, P.J., Steinman, H.M., Hill, R.M., and McKee, P.A.,J. Biol. Chem., 1974, vol. 249, pp. 1173–1181.

    PubMed  CAS  Google Scholar 

  35. Osterman, L.A.,Khromatografiya belkov i nukleinovykh kislot (Chromatography of Proteins and Nucleic Acids), Moscow: Nauka, 1985, pp. 348–349.

    Google Scholar 

  36. Fujkawa, K., Thompson, A.R., Legaz, M.E., Meyer, R.G., and Davie, E.W.,Biochemistry, 1973, vol. 12, pp. 4938–4945.

    Article  Google Scholar 

  37. Bradford, M.M.,Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  PubMed  CAS  Google Scholar 

  38. Laemmly, U.K.,Nature, 1970, vol. 227, pp. 680–685.

    Article  Google Scholar 

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch), Russian Academy of Sciences, 142292, Pushchino, Moscow oblast, Russia

    I. G. Shvyrkova & T. A. Muranova

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  1. I. G. Shvyrkova
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  2. T. A. Muranova
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Correspondence to T. A. Muranova.

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Shvyrkova, I.G., Muranova, T.A. Proteolytic specificity of plasmin toward adhesive proteins. Russ J Bioorg Chem 26, 317–321 (2000). https://doi.org/10.1007/BF02759283

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  • DOI: https://doi.org/10.1007/BF02759283

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