Abstract
The colony-forming units granulocyte and macrophage (CFU-GM) assay, using either rat or dog haematopoietic progenitor cells, assesses the toxicity of new compounds. To identify the characteristics of colony formation in this system, a time-course study of CFU-GM assays using rat and dog bone marrow cells was tested. Neutrophil colonies, macrophage colonies and mixed colonies of neutrophils and macrophages were formed in soft agar medium. Neutrophil colonies reached their maximum number on days 3–4 and decreased markedly thereafter. Macrophage colonies reached their maximum number on days 7–8 and remained steady thereafter. Only a small number of mixed colonies of neutrophils and macrophages were formed beginning around day 4. There were no significant differences between rat and dog bone marrow cells in the occurrence of these maxima, or in any other growth phenomenon. This result suggests that to evaluate the influence of compounds on neutrophil colonies and macrophage colonies, observations should be made on days 4 and 8, respectively.
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References
Bradley TR, Siemienowicz R (1968) Colony growth of rat bone marrow cells in vitro. Aust J Exp Biol Med Sci 46:595–605
Deldar A, Lewis H, Bloom J et al. (1988) Reproducible cloning assays for in vitro growth of canine hematopoietic progenitor cells and their potential applications in investigative hematotoxicity. Am J Vet Res 49:1393–1401
Deldar A, Stevens CE (1993) Development and application of in vitro models of hematopoiesis to drug development. Toxicol Pathol 21:231–240
Farris GM, Benjamin SA (1993) Inhibition of myelopoiesis by serum from dogs exposed to estrogen. Am J Vet Res 54:1374–1379
Gordon MY (1994) Stem cells and the microenvironment in aplastic anaemia. Br J Haematol 86:190–192
Gribaldo L, Bueren J, Deldar A et al. (1996) The use of in vitro systems for evaluating haematotoxicity. ATLA (Alternative to Laboratory Animals) 24:211–231
Hammond WP, Miller JE, Starkebaum G et al. (1988) Suppression of in vitro granulocytopoiesis by captopril and penicillamine. Exp Hematol 16:674–680
Kimura H, Finch CA, Adamson JW (1986) Hematopoiesis in the rat: quantitation of hematopoietic progenitors and the response to iron deficiency anemia. J Cell Physiol 126:298–306
Kubota K, Mizoguchi H, Miura Y et al. (1980) A new technique for the cytochemical examination of human hematopoietic cells grown in agar gel. Exp Hematol 8:339–344
Marsh JC, Levitt M, Katzenstein A (1972) The growth of leukocyte colonies in vitro from dog bone marrow. J Lab Clin Med 79:1041–1050
National Research Council (1996) Guide for the care and use of laboratory animals. National Research Council, National Academy Press, USA, Washington, DC
Parchment RE, Huang M, Erickson-Miller CL (1993) Roles for in vitro myelotoxicity test in preclinical drug development and clinical trial planning. Toxicol Pathol 21:241–250
Tan S, Miura Y, Motoyoshi K et al. (1981) Time course study of cell type in mouse granulocyte and macrophage colony formation. Acta Haematol Japon(JPN) 44:390 (abstr)
USDA (1989, 1991) 9 CFR Parts 1 and 2 Final Rules.
Volpe DA, Du D, Zurlo MG et al. (1992) Comparative in vitro myelotoxicity of FCE 24517, a distamycin derivative, to human, canine and murine hematopoietic progenitor cells. Invest New Drugs 10:255–261
Yamaguchi F, Furuhama K, Miyamoto M, et al. (1994) Application of hematopoietic progenitor assays for the estimation of hematotoxicity in rats. J Pharmacol Toxicol Methods 31:71–77
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Takamatsu, K., Wakata, A., Shishido, T. et al. The time course of leucocyte colony-formation in cultures of bone-marrow progenitor cells from rats and dogs. Comparative Haematology International 8, 191–196 (1998). https://doi.org/10.1007/BF02752847
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DOI: https://doi.org/10.1007/BF02752847