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Insulin-like growth factor I in human thyroid tissue: Specific localization by immunohistochemistry andIn Situ hybridization

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Abstract

Insulin-like growth factor I and II (IGF-I and IGF-II) have been implicated in the replication of normal thyroid follicular cells in vitro. This study evaluates the distribution and abundance of immunoreactive IGF-I by histochemical analysis in human thyroid tissue with different histopathologic characteristics. We used two types of highly specific and sensitive polyclonal rabbit anti-IGF-I antibodies and one monoclonal antibody (MAb) with the immunoperoxidase technique on sections of 25 glands harboring adenomatous goiter; 11 glands with follicular adenoma (FA); 45 glands with thyroid carcinoma of papillary, follicular, and undifferentiated types; and 18 glands with Graves' disease. Immunoreactive IGF-I was present in some thyroid follicular cells of all thyroid tissues examined. The percentage of cells staining positively varies among the different processes, being lowest in normal thyroid tissues and highest in all thyroid carcinomas. The cytoplasmic pattern of IGF-I immunoreactivity also varied among the different thyroid conditions. Furthermore, using nonradioactivein situ hybridization (ISH) we detected IGF-I mRNA in the thyroid cells of adenomatous goiter. The expression was higher in the histologically hyperplastic areas. These findings provide further support for an autocrine and/or paracrine role of IGF-I in the function and/or growth of normal thyroid follicular cells and suggest that IGF-I may play a role in the dysfunctional growth of thyroid follicular cells in adenomatous goiter, thyroid carcinoma, and Graves' hyperthyroidism.

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Author notes

  1. Edna T. Kimura MD, PhD

    Present address: Department of Histology and Cell Biology, Institute of Biomedical Sciences, University of São Paulo, Brazil

Authors and Affiliations

  1. Department of Pathology Escola Paulista de Medicina, Universidade Federal de São Paulo, Sao Paulo, Brazil

    Maria-Isabel S. Mesquita PhD & Vania N. Alberti MD, PhD

  2. Department of Medicine, Beth Israel Hospital and Haryard Medical School, Boston, MA

    Alan C. Moses MD

  3. Division of Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, Caixa Postal 20266, 04034-970, São Paulo, SP, Brazil

    Rui M. B. Maciel MD, PhD, Edna T. Kimura MD, PhD, Mirian H. Takahashi MD & Maria-Honorina C. Lopes MD, PhD

Authors
  1. Rui M. B. Maciel MD, PhD
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  2. Edna T. Kimura MD, PhD
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  3. Mirian H. Takahashi MD
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  4. Maria-Honorina C. Lopes MD, PhD
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  5. Maria-Isabel S. Mesquita PhD
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  6. Alan C. Moses MD
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  7. Vania N. Alberti MD, PhD
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Maciel, R.M.B., Kimura, E.T., Takahashi, M.H. et al. Insulin-like growth factor I in human thyroid tissue: Specific localization by immunohistochemistry andIn Situ hybridization. Endocr Pathol 6, 207–215 (1995). https://doi.org/10.1007/BF02739884

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