Abstract
The alterations in the lipid profiles of rat liver mitochondria due to vitamin A deficiency were studied. The amount of total lipids and phospholipids were decreased with a concomitant increase in triglycerides and cholesterol levels in mitochondria, isolated from vitamin A-deficient animals. Of particular significance was the observation that the content of lysolecithin, a potent cytolytic agent, was increased. An analysis of individual fatty acids showed that the percentage of polyunsaturated fatty acids was decreased significantly in vitamin A deficiency. Further, mitochondria from vitamin A-deficient animals, when incubated in 0.1 M Tris-HCl buffer (pH 7.4)in vitro, produced increased amounts of malondialdehyde and lipofuchsin pigments indicating increased susceptibility of the mitochondrial membrane to peroxidative damage. These results suggest a possible role of vitamin A in the prevention of the decomposition of structural lipids.
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Abbreviations
- TLC:
-
Thin layer chromatography
- GLC:
-
gas-liquid chromatography
- TBA:
-
thio-barbuturic acid
- PUFA:
-
polyunsaturated fatty acids
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Mullick, R.S., Adhikari, H.R. & Vakil, U.K. Changes in rat liver mitochondrial lipids in vitamin A deficiency. J Biosci 5, 243–251 (1983). https://doi.org/10.1007/BF02716607
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DOI: https://doi.org/10.1007/BF02716607