Abstract
A homogenous PreParation of Putrescine synthase, the versatile multifunctional enzyme involved in agmatine →Putrescine conversion inCucumis sativus was found to catalyze enzymatic decarboxylation of arginine also. Similarly, the Purified arginine decarboxylase mediated the comPonent as well as the comPlete set of couPled reactions harboured by Putrescine synthase. Both the enzyme PreParations exhibited identical electroPhoretic and chromatograPhic behaviour and were immunologically indistinguishable. All the enzymic activities are stabilized concurrently by feeding arginine to the intact seedlings. Therefore, it is concluded that the multifunctional Putrescine synthase inCucumis sativus seedlings also harbours arginine decarboxylase activity unlike its counterPart inLathyrus sativus.
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Abbreviations
- ADC:
-
Arginine decarboxylase
- PS:
-
Putrescine synthase
- AIHase:
-
agmatine iminohydrolase
- OTCase:
-
ornithine transcarbamylase
- PTCase:
-
Putrescine transcarbamylase
- CKase:
-
carbamate kinase
- PAGE:
-
Polyacrylamide gel electroPhoresis
- NCP:
-
N-carbamyl Putrescine
- SDS:
-
sodium dodecyl sulPhate
- ODC:
-
ornithine decarboxylase
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Prasad, G.L., Adiga, P.R. Arginine decarboxylase is a component activity of the multifunctional enzyme putrescine synthase in cucumber seedlings. J. Biosci. 11, 571–579 (1987). https://doi.org/10.1007/BF02704706
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DOI: https://doi.org/10.1007/BF02704706