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Construction of shuttle vectors for cloning inVibrio cholerae andEscherichia coli

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Abstract

Starting from a naturally occurring cryptic plasmid pVC540 ofVibrio cholerae non-OI. strain 1095, a number of plasmid vectors have been constructed for cloning genes inVibrio cholerae by introducing antibiotic resistance markers containing a set of unique cloning sites. The constructs pVC810 and pVE920 have the origins of bothVibrio cholerae andEscherichia coli replicons and are stable in both organisms in the absence of selective pressure. These plasmids can serve as shuttle vectors betweenEscherichia coli andVibrio cholerae. The plasmid vectors reported here along with the demonstration of transformation inVibrio cholerae by plasmid DNA will facilitate genetic analysis of this important human pathogen.

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Author notes

  1. Dibyendu K. Panda

    Present address: Molecular and Cell Biology Department, University of California, Cancer Research Laboratory, 447 Life Sciences Addition, 94720, Berkeley, California, USA

Authors and Affiliations

  1. Biophysics Division, Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, 700 032, Calcutta, India

    Dibyendu K. Panda, Rajat Banerjee & Jyotirmoy Das

Authors
  1. Dibyendu K. Panda
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  2. Rajat Banerjee
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  3. Jyotirmoy Das
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Panda, D.K., Banerjee, R. & Das, J. Construction of shuttle vectors for cloning inVibrio cholerae andEscherichia coli . J Biosci 20, 367–376 (1995). https://doi.org/10.1007/BF02703840

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  • DOI: https://doi.org/10.1007/BF02703840

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