Abstract
Rat liver lipoyl transacetylase catalyzes the formation of acetyl dihydrolipoic acid from acetyl coenzyme A and dihydrolipoic acid. In an earlier paper the formation of acetyl dihydrolipoic from pyruvate and dihydrolipoic acid catalyzed by pyruvate dehydrogenase has been reported. Acetyl dihydrolipoic acid is a substrate for citrate synthase, acetyl coenzyme A carboxylase and fatty acid synthetase. The Vmax. for citrate synthase with acetyl dihydrolipoic acid was identical to acetyl coenzyme A (approximately 1 μmol citrate formed/min/mg protein) while the apparent Km was approximately 4 times higher with acetyl dihydrolipoic acid as the substrate. This may be due to the fact that synthetic acetyl dihydrolipoic acid is a mixture of 4 possible isomers and only one of them may be the substrate for the enzymatic reaction. While dihydrolipoic acid can replace coenzyme A in the activation of succinate catalyzed by succinyl coenzyme A synthetase, the transfer of coenzyme A between succinate and acetoacetyl dihydrolipoic acid catalyzed by succinyl coenzyme A: 3 oxo-acid coenzyme A transferase does not occur.
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Abbreviations
- CoA:
-
Coenzyme A
- DTO:
-
dihydrolipoic acid
- DTNB:
-
5, 5 dithiobis (2-nitrobenzoic acid)
- BSA:
-
bovine serum albumin
- DTT:
-
dithiothreitol
- NAD:
-
nicotinamide adenine dinucleotide
- NADPH:
-
reduced nicotinamide adenine dinucleotide phosphate
- TLC:
-
thin layer chromatography
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Wagh, S.S., Gandhi, V.M., Natraj, C.V. et al. Lipoic acid and diabetes—Part III: Metabolic role of acetyl dihydrolipoic acid. J Biosci 10, 171–179 (1986). https://doi.org/10.1007/BF02703474
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DOI: https://doi.org/10.1007/BF02703474