Skip to main content
SpringerLink
Log in

Binding of thyroid hormone to mouse granulosa cell nuclei and its biological relevance

  • Published:
Journal of Biosciences Aims and scope Submit manuscript

Abstract

Thyroid hormone showed specific binding ability to mouse granulosa cells from immature mice, primed with post menopausal gonadotropin. Saturation of specific binding sites was reached by 2 nM concentration of the hormone. A Scatchard analysis of thyroid hormone binding exhibited a Kd of 42 x l0-9M/mg nuclear DNA and a maximum binding capacity of 1 pmol/mg nuclear DNA. Competitive inhibition studies showed thyroid hormone binding to be analogue specific. Addition of 100 ng of thyroid hormone to granulosa cell incubations (1 x 106 cells/well) resulted in a three-fold increase in cellular protein synthesis. Thyroid hormone resulted in a dose dependant increase in progesterone release from granulosa cell. It also stimulated the formation of pregnenolone (83%) and progesterone (81%) from radiolabeled cholesterol as compared to control. This stimulation by thyroid hormone was completely inhibited by cycloheximide. Results indicate a direct effect of thyroid hormone on granulosa cells, its binding to nuclei causing an increase in steroidogenesis through the mediation of protein(s).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akande E O 1975 Plasma estrogens in euthyroid and thyrotoxic women;Am. J. Obstet. Gynecol. 122 880–886

    Article  CAS  Google Scholar 

  • Arnott R D and Eastman C J 1983 Specific 3,3′, 5′-Triiodothyronine (reverse T3) binding sites on rat liver plasma membranes: Comparison with thyroxine (T4) binding sites;J. Receptor Res. 3 393–407

    Article  CAS  Google Scholar 

  • Ball J N 1960 Reproduction in female bony fishes;Symp. Zool. Soc. (London) 1 105–135

    Google Scholar 

  • Bernal J and DeGroot L 1980 Mode of action of thyroid hormones; inThyroid gland (ed.) M De Visscher (New York: Raven Press) pp 123–143

    Google Scholar 

  • Bhattacharya S, Banerjee J, Jamaluddin Md., Banerjee P P and Maitra G 1988 Thyroid hormone binds to human corpus luteum;Experientia 44 1005–1007

    Article  CAS  Google Scholar 

  • Chakraborti P and Bhattacharya S 1984 Plasma thyroxine levels in fresh water perch: Influence of season, gonadotropins, and gonadal hormones;Gen. Comp. Endrocrinol. 53 179–186.

    Article  CAS  Google Scholar 

  • Chakraborti P, Maitra G and Bhattacharya S 1986 Binding of thyroid hormone to isolated ovarian nuclei from a fresh water perch,Anabus testudineus;Gen. Comp. Endrocrinol. 62 239–246

    Article  CAS  Google Scholar 

  • Chandrasekher Y, D’Occhio M J and Setchell B P 1986 Reproductive hormone secretion and spermatogenic function in the thyroidectomised rams receiving graded doses of exogenous thyroxine ;J. Endrocrinol. 111 245–253

    Article  Google Scholar 

  • Cooke B A, Janszen F H A, Clotscher W F and Van Der Molen H J 1975 Effect of protein-synthesis inhibitors on testosterone production in rat testes interstitial tissue and leydig cell preparations;Biochem.J. 150 413–418

    Article  CAS  Google Scholar 

  • Dodd J M 1975 The hormones of sex and reproduction and their effects in fish and lower chrodates twenty years on;Am. Zool. (Suppl 1)15 127–171

    Google Scholar 

  • Gardner R M, Kirkland J L and Ireland J S 1978 Regulation of the uterine response to estrogen by thyroid hormone;Endrocrinology 103 1168–1172

    Google Scholar 

  • Gorbman A, Dickhoff W W, Vigna S R, Clark M B and Ralph C L 1983 The reproductive system; inComparative endocrinology (New York, Chichester, Brisbane, Toronto, Singapore: Wiley Interscience) pp 255–256

    Google Scholar 

  • Gordon G G and Southren A L1977 Thyroid hormone effects on steroid hormone metabolism;N. Y. Acad. Med. 53 241–259

    CAS  Google Scholar 

  • Greenwald G S and Limback D 1984 Effects of treatment with cycloheximide atproestrus on subsequentin vitro follicular steroidogenesis in hamster;Biol. Reprod. 30 1105–1116

    Article  CAS  Google Scholar 

  • Howland B E and Ibrahim E A 1973 Hyperthyroidism and gonadotropin secretion in male and female rats;Experientia 29 1398–1399

    Article  CAS  Google Scholar 

  • Ingbar S H and Woebar K A 1981 The thyroid gland; inText book of endrocrinology (ed.) R H Williams (Philadelphia, London, Toronto: WB Saunders)pp 117–247

    Google Scholar 

  • Jannini E A, Olivieri M, Fancavilla S, Gulino A, Ziparo E and D’Armiento M 1990 Ontogenesis of the nuclear 3,3′, 5-triiodothyronine receptor in the rat testis;Endocrinology 26 2521–2525

    Article  Google Scholar 

  • Kato R, Onoda K and Omori Y 1970 Mechanism of thyroid induced increase in steroid δ-4-reductase activity in male rats;Endocrinol. Jpn. 17 215

    Article  CAS  Google Scholar 

  • Kaul K and Bhattacharya S 1988 Thyroid hormone stimulation of the perch(Anabus testudineus Bloch) ovarian mitochondrial steroidogenesis;Indian J. Exp. Biol. 26 413–417

    CAS  PubMed  Google Scholar 

  • Kirkland J L, Gardner R M and Mukku V R 1981 Hormonal control of uterine growth: The effect of hypothyroidism on estrogen stimulated cell division;Endocrinology 108 2346–2351

    Article  CAS  Google Scholar 

  • Kirkland J L, Mukku V R, Hardy M and Young R 1983 Effects of triiodothyronine receptors in human endometrium and myometrium;Am. J. Obstet. Gynecol. 146 380–383

    Article  CAS  Google Scholar 

  • Koerner D, Schwartz H L, Surks M I, Oppenheimer J H and Jorgensen E C 1975 Binding of selected Iodothyronine analogues to receptor sites in isolated rat hepatic nuclei;J. Biol. Chem. 250 6417–6423

    CAS  PubMed  Google Scholar 

  • Korenman S G, Stevens R H, Carpenter L A, Robb M, Niswender G D and Sherman B M 1974 Estradiol radioimmunoassay without chromatography: Procedure validation and normal values;J. Clin. Endocrinol. Metab. 38 718–720

    Article  CAS  Google Scholar 

  • Larson P R and Ingbar S H 1992 The thyroid gland; inWilliam’s textbook of endocrinology 8th edition (eds) J D Wilson and D W Foster (Philadelphia, London, Toronto, Montreal, Sydney, Tokyo: W B Saunders) pp 357–487

    Google Scholar 

  • Latham K R, Ring J C and Baxter J D 1976 Solubilized nuclear “receptor” for thyroid hormones;J. Biol. Chem. 251 7388–7397

    CAS  PubMed  Google Scholar 

  • Lawson G M, Ti, M-J, Tsai, S Y, Minghetti P P, McClure M E and O’Malley B W 1987Laboratory methods manual for hormone action and molecular endocrinology (Houston Biological Assoc, USA) pp 71–732

    Google Scholar 

  • Maitra G and Bhattacharya S 1989 Seasonal changes of triiodothyronine binding to piscine ovarian nuclei;Zool Sci. 6 771–776

    CAS  Google Scholar 

  • May J V and Schomberg D W 1981 Granulosa cell differentiationin vitro: effect of insulin on growth and functional integrity;Biol. Reprod. 25 421

    Article  CAS  Google Scholar 

  • McLeod K M and Baxter J D 1976 Chromatin receptors for thyroid hormone: Interactions of the solubilized proteins with DNA;J. Biol. Chem. 251 7380–7387

    Google Scholar 

  • Morgan G 1984 Thyroid hormone binding in rat liver cytosol;J. Endocrinol. 103 265–271

    Article  CAS  Google Scholar 

  • Mukku V R, Kirkland J L, Hardy M and Stancel G 1983 Evidence for thyroid hormone receptors in uterine nuclei;Metabolism 2 142–145

    Article  Google Scholar 

  • Oppenheimer J H 1979 Thyroid hormone action at the cellular level;Science 203 971–979

    Article  CAS  Google Scholar 

  • Sage M 1973 The evolution of thyroidal function in fishes;Am. Zool. 13 899–905

    Article  CAS  Google Scholar 

  • Scatchard G 1949 The attraction of proteins for small molecules;Ann. N. Y. Acad. Sci. 51 660–672

    Article  CAS  Google Scholar 

  • Schreider G, Kopach K, Chanian H, Bonnefond V, Mittler J C and Ertel N H 1979 The Hypothalamic-pituitary-gonadal axis during hypothyroidism in the rat;Endocrinology 105 674

    Article  Google Scholar 

  • Seelig S, Liaw C, Towle S C and Oppenheimer J H 1981 Thyroid hormone attenuates and augments hepatic gene expression at a pretranslational level;Proc. Natl. Acad. Sci. USA 78 4733–4737

    Article  CAS  Google Scholar 

  • Sen S and Bhattacharya S 1982 Hormonal influence on perch ovarian 17 Β-Hydroxysteroid dehydrogenase activity inin vitro system;Indian J. Exp. Biol. 20 664–667

    CAS  PubMed  Google Scholar 

  • Snedecor G W and Cochran W G 1968Statistical methods (Ames: Iowa State University Press)

    Google Scholar 

  • Southren A C, Olivio J, Gordon G G, Vittek J, Briner J and Rafii F 1974 The conversions of androgens to estrogens in hyperthyroidism;J. Clin. Endrocrinol Metab. 38 207–214

    Article  CAS  Google Scholar 

  • Sterling K and Milch P O 1975 Thyroid hormone binding by a component of mitochondrial membrane;Proc. Natl. Acad. Sci. USA 72 3225–3229

    Article  CAS  Google Scholar 

  • Surks M I, Koerner D H, Dillman W and Oppenheimer J H 1973 Limited capacity binding sites for L-triiodothyronine in rat liver nuclei;J. Biol. Chem. 248 7066–7072

    CAS  PubMed  Google Scholar 

  • Tata J R 1974 Growth and developmental action of thyroid hormones at the cellular level; inHandbook of physiology Section VII, Vol. 3 “TheThyroid”, (eds) M A Greer and D S Solomon (Washington DC: American Physiological Society) pp 469–478

    Google Scholar 

  • Tsafriri A, Lieberman M E, Barnea A, Bauminger S and Linder H R 1973 Induction by luteinizing hormone of ovum maturation and steroidogenesis in isolated graafian follicles of rat: Role of RNA and protein synthesis;Endrocrinology 93 1378–1386

    Article  CAS  Google Scholar 

  • Wakim N G, Ramani N and Rao Ch V 1987 Triiodothyronine receptors in porcine granulosa cells;Am. J. Obstet. Gynecol. 156 237–240

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, Visva-Bharati University, 731 235, Santiniketan, India

    R. Biswas, A. Bandyopadhyay, S. Guin & S. Bhattacharya

Authors
  1. R. Biswas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Bandyopadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Guin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Bhattacharya
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Biswas, R., Bandyopadhyay, A., Guin, S. et al. Binding of thyroid hormone to mouse granulosa cell nuclei and its biological relevance. J. Biosci. 18, 327–335 (1993). https://doi.org/10.1007/BF02702990

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02702990

Keywords

Search

Navigation