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Long-lived southern blots using RNA probes

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Abstract

Conditions for preparation and hybridization of Southern blots are described which assure reusability through 15 to 25 cycles. The procedure relies on the use of charge-modified nylon membranes and[32P]-labelled RNA probes.

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Authors and Affiliations

  1. Agrigenetics Company, 5649 East Buckeye Road, 53716, Madison, Wisconsin, U.S.A.

    Michael G. Murray, Jan W. Pitas, Sandra J. DeMars & Jane Harris Cramer

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  1. Michael G. Murray
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  2. Jan W. Pitas
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  3. Sandra J. DeMars
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  4. Jane Harris Cramer
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Murray, M.G., Pitas, J.W., DeMars, S.J. et al. Long-lived southern blots using RNA probes. Plant Mol Biol Rep 10, 173–177 (1992). https://doi.org/10.1007/BF02668344

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  • DOI: https://doi.org/10.1007/BF02668344

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