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Isolation and long-term maintenance of differentiated adult chicken hepatocytes in primary culture

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Summary

Adult chicken hepatocytes were obtained by an adaptation of the two step in situ collagenase perfusion. Usually 0.5 to 1 × 109 cells were obtained, with 75 to 95% viability. Hepatocytes attached within 2 h when plated on plastic cell culture dishes and spread in 4 h, surviving for several months in a specific serum-free medium. These cells retained a typical parenchymal cell morphology and the ability to produce a specific protein (albumin) throughout the culture period. We hereby provide a suitable model for studying hepatic metabolism in birds.

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Fraslin, JM., Touquette, L., Douaire, M. et al. Isolation and long-term maintenance of differentiated adult chicken hepatocytes in primary culture. In Vitro Cell Dev Biol - Animal 28, 615–620 (1992). https://doi.org/10.1007/BF02631036

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