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Primary cultures of embryonic rat heart cells

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Summary

In contrast to neonatal rat heart cells in culture, embryonic myocardial cells form colonies. They also differ from neonatal cells in their appearance and behavior; they have fewer striations, fewer binucleated cells, loose packing and sorting from nonmuscle cells, and most of all a greater capacity to divide.

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References

  1. Geynai, A. Relation between spontaneous activity and cholinesterase activity in dissociated embryonic and young rat heart and embryonic chick heart cells studied in tissue culture. Histochimie 18: 150–156; 1969.

    Article  Google Scholar 

  2. Harary, I.; Hoover, F.; Farley, B. The isolation and cultivation of rat heart cells. Fleischer, S.; Packer, L. eds. Methods in enzymology. New York: Academic Press; 1974: 740–745.

    Google Scholar 

  3. Healy, G. M.; Parker, W. C. An improved chemically defined basal medium (CMRL 1415) for newly explanted mouse embryos. J. Cell Biol. 30: 531–538; 1966.

    Article  PubMed  CAS  Google Scholar 

  4. Clark, W. A. Selective control of fibroblast proliferation and its effect on cardiac muscle differentiation in vitro. Dev. Biol. 52: 263–282; 1976.

    Article  PubMed  CAS  Google Scholar 

  5. Goode, D. Mitosis of embryonic heart muscle in vitro. An immunofluorescence and ultrastructural study. Cytobiology 11: 203–229; 1975.

    CAS  Google Scholar 

  6. Chacko, S. DNA synthesis, mitosis and differentiation in cardiac myogenesis. Dev. Biol. 35: 1–18; 1973.

    Article  PubMed  CAS  Google Scholar 

  7. De Haan, R. L. Regulation of spontaneous activity and growth of embryonic chick heart cells in tissue culture. Dev. Biol. 16: 216–249; 1967.

    Article  Google Scholar 

  8. Polinger, I. Growth and DNA synthesis in embryonic chick heart cells, in vivo and in vitro. Exp. Cell Res. 76: 253–262; 1973.

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. Marie J. O. Masse

    Present address: Groupe de Neurobiochimie Cellulaire et Moleculaire, Universite Pierre et Marie Curie, 96 Boulevard Raspail, 75006, Paris, France

Authors and Affiliations

  1. Laboratory of Nuclear Medicine and Radiation Biology, University of California, 90024, Los Angeles, California

    Marie J. O. Masse & Isaac Harary

  2. Molecular Biology Institute, University of California, 90024, Los Angeles, California

    Marie J. O. Masse & Isaac Harary

  3. Department of Biological Chemistry, UCLA School of Medicine, 90024, Los Angeles, California

    Marie J. O. Masse & Isaac Harary

Authors
  1. Marie J. O. Masse
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  2. Isaac Harary
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Additional information

This work was supported in part by Department of Energy Contract EY-76-C-03-0012 and Environmental Protection Agency Contract IAG-D5-E-681-AO.

This paper is part of a dissertation submitted by M. J. O. M. in partial fulfillment of the requirement for the Ph.D. degree at the University of California.

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Masse, M.J.O., Harary, I. Primary cultures of embryonic rat heart cells. In Vitro 17, 388–392 (1981). https://doi.org/10.1007/BF02626737

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  • DOI: https://doi.org/10.1007/BF02626737

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