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Somatic embryony patterns and plant regeneration inIpomoea batatas poir

  • Plant Cellular and Developmental Biology
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Summary

Patterns of somatic embryo development from callus were elucidated inIpomoea batatas Poir. (sweet potato). Embryos at three stages of development were able to grow into plants. They included embryos arrested at the late torpedo and cotyledonary stages, and an arrested torpedo embryo that resulted from precocious hypocotyl expansion. Early torpedo-stage embryos rooted on reculture but did not form shoots. A diversity of other embryo stages were also produced which upon reculture formed only adventitious embryos and roots. Morphologic variants with similar growth potential were noted among embryos at similar stages of development. Plasticity in somatic embryo developmental patterns led to different forms that were mature enough to produce plants.

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References

  1. Ammirato, P. V. Patterns of development in culture. In: Henke, R. R.; Hughes, K. W.; Constantin, et al. eds. Tissue culture in forestry and agriculture. New York: Plenum Press; 1985:9–29.

    Google Scholar 

  2. Ammirato, P. V.; Steward, F. C. Some effects of the environment on the development of embryos from cultured free cells. Bot. Gaz. 132:149–158; 1971.

    Article  Google Scholar 

  3. Dos Santos, A. V. P.; Cutter, E. G.; Davey, M. R. Origin and development of somatic inMedicago sativa L. (Alfalfa). Protoplasma 117:107–115; 1983.

    Article  Google Scholar 

  4. Gray, D. J.; Conger, B. V. Time-lapse light photomicrography and scanning electron microscopy of somatic embryo ontogeny from cultured leaves ofDactylis glomerata (Gramineae). Trans. Am. Microsc. Soc. 104:395–399; 1985.

    Article  Google Scholar 

  5. Haccius, B. Question of unicellular origin of non-zygotic embryos in callus cultures. Phytomorphology 28:74–81; 1978.

    Google Scholar 

  6. Haccius, B.; Bhandari, N. N. Delayed histogen differentiation as a common primitive character in all types of non-zygotic embryos. Phytomorphology 25:91–94; 1975.

    Google Scholar 

  7. Halperin, W. Alternative morphogenetic events in cell suspensions. Am. J. Bot. 53:443–453; 1966.

    Article  Google Scholar 

  8. Jarret, R. L.; Salazar, S.; Fernandez, Z. R. Somatic embryogenesis in sweet potato. HortScience 19:397–398; 1984.

    Google Scholar 

  9. Konar, R. N.; Thomas, E.; Street, H. E. The diversity of morphogenesis in suspension cultures ofAtropa belladonna L. Ann. Bot. 36:249–258; 1972.

    Google Scholar 

  10. Liu, J. R.; Cantliffe, D. J. Somatic embryogenesis and plant regeneration in tissue culture of sweet potato (Ipomoea batatas Poir.). Plant. Cell. Rep. 3:112–115; 1984.

    Article  Google Scholar 

  11. Lutz, J. D.; Wong, J. R.; Rowe, J., et al. Somatic embryogenesis for mass cloning in crop plants. In: Henke, R. R.; Hughes, K. W.; Constantin, M. J., et al. eds. Tissue culture in forestry and agriculture. New York: Plenum Press; 1985:105–116.

    Google Scholar 

  12. Murashige, T.; Skoog, F. A revised media for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  13. Pierson, P.; Koehler, S.; Wright, M. Histology of structures from a soybean (Glycine max) somatic embryogenesis protocol. In: Henke, R. R.; Hughes, K. W.; Constantin, M. J., et al., eds. Tissue culture in forestry and agriculture. New York: Plenum Press; 1985:341.

    Google Scholar 

  14. Stuart, D. A.; Nelsen, J.; Strickland, S. G., et al. Factors affecting developmental processes in alfalfa cell cultures. In: Henke, R. R.; Hughes, K. W.; Constantin, M. J., et al., eds. Tissue culture in forestry and agriculture. New York: Plenum Press; 1985:59–73.

    Google Scholar 

  15. Tsay, H.-S.; Tseng, M.-T. Embryoid formation and plantlet regeneration from anther callus of sweet potato. Bot. Bull. Academia Sinica 20:117–122; 1979.

    Google Scholar 

  16. Vasil, I. K.; Hildebrandt, A. C. Variations of morphogenetic behavior in plant tissue cultures II.Petrolinum hortense. Am. J. Bot. 53:869–874; 1966.

    Article  Google Scholar 

  17. Vasil, V.; Vasil, I. K. The ontogeny of somatic embryos ofPenisetum americanum (L.) K. Schum. I. in cultured immature embryos. Bot. Gaz. 143:454–465; 1982.

    Article  Google Scholar 

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Authors and Affiliations

  1. Vegetable Crops Department, University of Florida, Institute of Food and Agricultural Sciences, 32611, Gainesville, Florida

    Raymond P. Chée & Daniel J. Cantliffe

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  1. Raymond P. Chée
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  2. Daniel J. Cantliffe
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This work has been funded in part by an IFAS/Gas Research Institute cooperative grant. Florida Agricultural Experiment Station Journal Series No. 8789.

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Chée, R.P., Cantliffe, D.J. Somatic embryony patterns and plant regeneration inIpomoea batatas poir. In Vitro Cell Dev Biol 24, 955–958 (1988). https://doi.org/10.1007/BF02623910

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  • DOI: https://doi.org/10.1007/BF02623910

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