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Collagen synthesis in normal BHK cells and temperature-sensitive chemically transformed BHK cells

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Summary

Collagen synthesis in normal BHK 21/cl 13 and chemically transformed temperature-sensitive BHK 21/cl 13 cells (Me2N4) was assessed by examination of hydroxyproline formation and collagenase-susceptible protein. The Me2N4 cells lost their ability to synthesize collagen at both permissive and nonpermissive temperatures for transformation. These conclusions were confirmed by polyacrylamide-gel electrophoresis and CM-cellulose chromotography. Prolyl hydroxylase activity was present in both normal and transformed cells even when no collagen could be demonstrated. The production of noncollagen protein, although decreased in the transformed cell, did not change as drastically as the collagen synthesis.

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Authors and Affiliations

  1. Departments of Biochemistry and Microbiology, Boston University School of Medicine, 02118, Boston, Massachusetts

    D. Biles, W. Gonnerman, B. Faris, A. Levine, N. Capparell, F. Moolten & C. Franzblau

  2. Connective Tissue-Aging Research Laboratory, Veterans Administration Outpatient Clinic, 17 Court Street, 02108, Boston, Massachusetts

    B. D. Smith

Authors
  1. B. D. Smith
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  2. D. Biles
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  3. W. Gonnerman
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  4. B. Faris
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  5. A. Levine
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  6. N. Capparell
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  7. F. Moolten
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  8. C. Franzblau
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Additional information

This paper was supported in part by a grant from the Public Health Service (AG00001), and by the Medical Research Service of the Veterans Administration.

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Smith, B.D., Biles, D., Gonnerman, W. et al. Collagen synthesis in normal BHK cells and temperature-sensitive chemically transformed BHK cells. In Vitro 15, 455–462 (1979). https://doi.org/10.1007/BF02618415

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  • DOI: https://doi.org/10.1007/BF02618415

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