Summary
Lung organ bits taken from full-term mice were explanted on the dermal surface of sterile, dead pigskin. The cells migrated onto the pigskin dermis and proliferated to form an organoid culture consisting of ductular structures separated by a matrix of epithelial cells. Cells within the ductular structures were ciliated, produced mucin, and exhibited the activities of nonspecific esterase and gamma-glutamyl transferase; therefore they were considered to be derived from bronchial epithelium. Cells forming the matrix possessed the activities of nonspecific esterase and alkaline phosphatase and contained lamellar structures typical of surfactant-producing pneumocyte Type II cells; therefore they were considered to be derived from alveolar precursor cells.
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Doll, R.; Hill, A. B. Mortality of doctors in relation to their smoking habits. Preliminary Report. Br. Med. J. I: 1451–1455; 1954.
Hammond, E. C.; Horn, D. Relationship between human smoking habits and death rates; Followup study of 187, 766 Men. J. Am. Med. Assoc. 155: 1316–1328; 1954.
Lasnitzki, I. The effect of 3–4 benzypyrene on human foetal lung grown in vitro. Br. J. Cancer 10: 510–516; 1956.
Lasnitzky, I. Observation on human foetal lung in vitro. Br. J. Cancer 12: 547–552; 1958.
Crocker, T. T.; O'Donnell, T. V.; Nunes, L. L. Toxicity of benzo(a)pyrene and air pollution composite for adult human bronchial mucosa in organ culture. Cancer Res. 33: 88–93; 1973.
Marchok, A. C.; Cone, M.; Nettesheim, P. Induction of squamous metaplasia (vitamin A deficienc) and hypersecretory activity in tracheal organ cultures. Lab. Invest. 33: 451–460; 1975.
Lane, B. P.; Gordan, R. Regeneration of rat tracheal epithelium after mechanical injury. I. The relationship between mitotic activity and cellular differentiation. Proc. Soc. Exp. Biol. Med. 145: 1139–1144; 1974.
Leuchtenberger, C.; Leuchtenberger, R. Cytologic and cytochemical effects on primary mouse kidney tissue and lung organ cultures after exposure to whole, fresh smoke and its gas phase from unfiltered, charcoal-filtered, and cigar tobacco cigarettes. Cancer Res. 29: 862–872, 1969.
Crocker, T. T.; Nielsen, B. L.; Lasnitzky, I. Carcinogenic hydrocarbons: Effects on suckling rat trachea in organ culture. Arch. Environ. Health 10: 240–250; 1965.
Dirksen, E. R.; Crocker, T. T. Ultrastructural alterations produced by polycyclic aromatic hydrocarbons on rat tracheal epithelium in organ culture. Cancer Res. 28: 906–923; 1968.
Marchok, A. C.; Rhoton, J.; Griesemer, R. A.; Nettesheim, P. Increased in vitro growth capacity of tracheal epithelium exposed in vivo to 7–12 dimethylbenz(a)anthracene Cancer Res. 37: 1811–1821; 1977.
Indo, K.; Wilson, R. B. Cell interactions between epithelial and mesenchymal components of primary sheets of fetal rat lung cells, and the effects of 3-methylchelanthrene treatment. J. Natl. Cancer Inst. 57: 1333–1339; 1976.
Indo, K. Biologic analysis of fetal MRC rat lung epithelial cells treated with 3-methylchelanthrene in culture: Premalignant and malignant stages. J. Natl. Cancer Inst. 58: 351–360; 1977.
Laws, J. O.; Flaks, A. Pulmonary adenomata induced by carcinogen treatment in organ culture. Br. J. Cancer 20: 550–554; 1966.
Trowell, G. A. A modified technique for organ culture in vitro. Exp. Cell Res. 6: 246–248; 1954.
Steele, V. E.; Marchok, A. C.; Nettesheim, P. Transformation of tracheal epithelium exposed in vitro toN-methyl-N-nitro-N-nitrosoguanidine (MNNG). Int. J. Cancer 20: 234–238; 1977.
Frosolono, M. F.; Kress, Y.; Wittner, M.; Rosenbaum, R. M. Culture characteristics of cells derived from Type II pneumocyte enriched fractions from rabbit and rat. In Vitro 12: 708–717; 1976.
Douglas, W. H. J.; Kaighn, M. E. Clonal isolation of differentiated rat lung cells. In Vitro 10: 230–237; 1974.
Douglas, W. H. J.; Hitchcock, K. R. Organotypic cultures of diploid Type II alveolar pneumocytes surfactant associated esterase activity. J. Histochem. Cytochem. 27: 852–856; 1979.
Freeman, A. E.; Igel, H. J.; Waldman, N. L.; Losikoff, A. M. A new method for covering large surface area wounds with autografts. I. In vitro multiplication of rabbit-skin epithelial cells. Arch. Surg. 108: 721–723; 1974.
Freeman, A. E.; Igel, H. J.; Herrman, B. J.; Kleinfeld, K. L. Growth and characterization of human skin epithelial cell cultures In Vitro 12; 352–362; 1976.
Burstone, M. S.; and Keyes, P. H. Studies on calcification. I. The effects of inhibition of enzyme activity on developing bone and dentin. Am. J. Pathol. 13: 1229–1235; 1957.
Burstone, M. S. The cytochemical localization of esterase. J. Natl. Cancer Inst. 18: 167–172; 1957.
Tanaka, M. A histochemical study on the activity of gamma-glutamyl transpeptidase in liver disease. Acta Pathol. Jpn. 24: 651–665; 1974.
Freeman, A. E.; Lake, R. S.; Igel, H. J.; Gernand, L.; Pezzutti, M. R.; Malone, J. M.; Mark, C.; and Benedict, W. F. Heteroploid conversion of human skin cells by methylcholanthrene. Proc. Natl. Acad. Sci. U.S.A. 74: 2451–2455; 1977.
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This research was supported by Grant-in-Aid 1203 M from the Council for Tobacco Research, awarded to Aaron E. Freeman.
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Yoshida, Y., Hilborn, V., Hassett, C. et al. Characterization of mouse fetal lung cells cultured on a pigskin substrate. In Vitro 16, 433–445 (1980). https://doi.org/10.1007/BF02618367
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DOI: https://doi.org/10.1007/BF02618367