Summary
A cell cycle analysis of theTrichoplusia ni (TN-368) insect cell line is described. By means of autoradiography and percent labeled metaphase data, the cell cycle parameters were determined to be as follows: S, 4.5 hr; G2, 8.5 hr; M, 0.5 hr; G1, 1.0 hr; the total cell time being 14.5 hr. A synchronization procedure using 50mm thymidine in a double block procedure was used to provide a method of obtaining a large number of cells in particular cell cycle phases, especially S and G2.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hink, W. F. 1970. Established insect cell line from the cabbage looper,Trichoplusia ni. Nature 226: 466–467.
Hink, W. F., and E. Strauss. 1976. Replication of alfalfa looper nuclear polyhedrosis virus in theTrichoplusia ni. (TN-368) cell line. In: E. Kurstak, and K. Maramorosch (Ed.),Invertebrate Tissue Culture, Applications in Medicine, Biology, and Agriculture. Academic Press, New York, pp. 369–374.
Stubblefield, E. 1968. Synchronization methods for mammalian cell cultures. In: D. M. Prescott (Ed.),Methods in Cell Physiology. Vol. 3. Academic Press, New York, pp. 25–43.
Puck, T. T. 1964. Phasing, mitotic delay, and chromosomal aberrations in mammalian cells. Science 144: 565–566.
Quastler, H., and F. G. Sherman. 1959. Cell population kinetics in the intestinal epithelium of the mouse. Exp. Cell Res. 17: 420–438.
Engelberg, J. 1964. Measurement of degrees of synchrony in cell populations. In: E. Zeuthen (Ed.),Synchrony in Cell Division and Growth. Interscience Publishers. New York, pp. 497–508.
Dolfini, S., A. M. Courgeon, and L. Tiepolo. 1970. The cell cycle of an established line ofDrosophila melanogaster cellsin vitro. Experientia 26: 1020–1021.
Miltenburger, H. G., P. David, and H. Wulf. 1976. Establishment ofMamestra brassicae cell lines and NPV-replication.Proceedings 1st International Colloquium on Invertebrate Pathology. Queen's University Press, Kingston, Canada, pp. 393–394.
Cleaver, J. E. 1967.Thymidine Metabolism and Cell Kinetics. North Holland Research Monographs, Amsterdam.
Mitchison, J. M. 1971.The Biology of the Cell Cycle. Cambridge Press, Cambridge.
Ooka, T. 1976. A method for synchronization of animal cells in culture. In: D. M. Prescot (Ed.),Methods in Cell Biology. Vol. 14. Academic Press, New York, pp. 287–295.
Tobey, R. A., and H. A. Crissman. 1972. Preparation of large quantities of synchronized animal cells in late G1 of the pre-DNA replicative phase of the cell cycle. Exp. Cell Res. 75: 460–464.
Author information
Authors and Affiliations
Additional information
This work was supported in part by U.S. Environmental Protection Agency Grant R-802516.
Rights and permissions
About this article
Cite this article
Lynn, D.E., Hink, W.F. Cell cycle analysis and synchronization of the TN-368 insect cell line. In Vitro 14, 236–238 (1978). https://doi.org/10.1007/BF02618228
Issue Date:
DOI: https://doi.org/10.1007/BF02618228