Skip to main content
SpringerLink
Log in

Divergen properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

Two lymphocytic cell lines were obtained from a single specimen of peripheral blood buffy coat from a patient with acute lymphoblastic leukemia who concurrently developed classical infectious mononucleosis. These two cell lines exhibit distinguishing differences in culture in parallel with reviously described differences in the cells when implanted into eonatal Syrian hamsters. These observations suggest that the cell lines were derived from two pre-existing different classes or “stelines” of cells—one of which resembles other cell lines derived from patients with acute lymphoblastic leukemia, whereas the other resembles cell lines derived from other, patients with infecttious mononucleosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Benyesh-Melnick, M., D. J. Fernbach, and R. T. Lewis. 1963. Studies on human leukmia. I. Spontaneous lymphoblastoid transformation of fibroblastic bone marrow cultures derived from leukemic and nonleukemic children. J. Natl. Cancer Inst. 31: 1311–1325.

    PubMed  CAS  Google Scholar 

  2. Foley, G. E, H. Lazarus, S. Farber, B. G. Uzman, B. A. Boone, and R. E. McCarthy. 1965. Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522–529.

    Article  PubMed  CAS  Google Scholar 

  3. Jensen, E. M., W. Korol, S. L. Dittmar, and T. J. Medrek. 1967. Virus containing lymphocyte cultures from cancer patients. J. Natl. Cancer Inst. 39: 745–754.

    PubMed  CAS  Google Scholar 

  4. Moore, G. E., J. T. Gracc, Jr., P. Citron, R. Gerner, and A. Burns. 1966. Lekocytecultures of patients with, leukemia and lymphomas. N. Y. State J. Med. 66: 2757–2764.

    PubMed  CAS  Google Scholar 

  5. Lazarus, H., and G. E. Foley. 1972. Cultivation of hematopoietic cells. In: G. H. Rothblat. and V. J. Cristofalo (Eds.),Growth, Nutrition and Metabolism of Cells in Culture. Volume II, Chapter 5. Academic Press, New York, pp. 170–212.

    Google Scholar 

  6. Adams, R. A., L. Pothier, A. Flowers, H. Lazarus, S. Farber, and G. E. Foley. 1970. The question of stem-lines in human acute leukemia: Comparison of cells islated in vitro and in vivo from a patient with acute lymphoblastic leukemia. Expt. Cell Res. 62: 5–10.

    Article  CAS  Google Scholar 

  7. Adams, R. A., A. Flowers, and B. J. Davis. 1968. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121–1125.

    PubMed  CAS  Google Scholar 

  8. Foley, G. E., H. Lazarus, S. Farber, B. G. Uzman, and R. A. Adams. 1968. Studies on human leukemic cells in vitro. In:The Proliferation and Spread of Neoplastic Cells. 21st Annual Symposium on Fundamental Cancer Research, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, TX. The Williams & Wilkins Co., Baltimore, pp. 65–97.

    Google Scholar 

  9. Eagle, H. 1959. Amino acid metabolism in mammalian cell cultures. Science 30: 432–437.

    Article  Google Scholar 

  10. Uzman, B. G., G. E. Foley, S. Farber, and H. Lazarus. 1966. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term cuture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725–1742.

    Article  PubMed  CAS  Google Scholar 

  11. Friedkin, M., and D. Roberts. 1954. The enzymatic synthesis of nucleotides. I. Thymidine phosphorylase in mammalian tissue. J. Biol. Chem. 207: 245–256.

    PubMed  CAS  Google Scholar 

  12. Marsh, J. C., and S. Perr. 1964. Thyidine catabolism by normal and leukemic human leukocytes. J. Clin. Invest. 43: 267–278.

    Article  PubMed  CAS  Google Scholar 

  13. Friedemann, T. E., and G. E. Haugen. 1943. Pyruvic acid. II. The determination of keto acids in blood and urine. J. Biol. Chem. 147: 415–442.

    CAS  Google Scholar 

  14. Sayre, F. W., and D. M. Greenberg. 1956. Purification and properties of serine and threonine dehydrases. J. Biol. Chem. 220: 787–799.

    PubMed  CAS  Google Scholar 

  15. Matsuo, Y., and D. M. Greenberg. 1958. A crystalline enzyme that cleaves homoserine and cystathionine. I. Isolation procedure and some physicochemical properties. J. Biol. Chem. 230: 545–560.

    PubMed  CAS  Google Scholar 

  16. Oyama, V. I., and H. Eagle. 1956. Measurment of cell growth in tissue culture with a pheno regent (Folin-Ciocalteau). Proc. Soc. Expt. Biol. Med. 91: 305–307.

    CAS  Google Scholar 

  17. Uzman, B. G., and A. Krishan. 1969. Virus or virus-derived aggregates in cells cultured from leukemia and infectious mononucleosis. Proc. Am. Assoc. Cancer Res. 10: 95.

    Google Scholar 

  18. Moses, H. L., P. R. Glade, J. A. Kasel, A. S. Rosenthal, Y. Hirshaut, and L. N. Chessin. 1968. Infectious mononucleosis: Detection of herpes-like virus and reticular aggregates of small cytoplasmic particles in cotinuous lymphoid cell lines derived from peripheral blood. Proc. Natl. Acad. Sci. U.S.A. 60: 489–496.

    Article  PubMed  CAS  Google Scholar 

  19. Chandra, S., G. E. Moore, and P. M. Brandt. 1968. Similarity between leukocyte cultures from cancerous and noncancerous human subjects: An electronmicroscopic study. Cancer Res. 28: 1982–1989.

    PubMed  CAS  Google Scholar 

  20. Uzman, B. G., H. Saito, and M. Kasac. 1971. Tubular arrays in the endoplasmic reticulum in human tumor cells. Lab. Invest. 24: 492–498.

    PubMed  CAS  Google Scholar 

  21. Schaff, Z., U. Heine, and A. J. Dalton. 1972. Ultramorphological and ultracytochemical studies on tubuloreticular structures in lymphoid cells. Cancer Res. 32: 2696–2706.

    PubMed  CAS  Google Scholar 

  22. Foley, G. E., E. F. Barell, R. A. Adams, and H. Lazarus. 1969. Nutritonal requirements of human leukemic cells: Cystine requirements of diploid cell lines and their heteroploid variants. Exp. Cell Res. 57: 129–133.

    Article  PubMed  CAS  Google Scholar 

  23. Schachtschabel, D. O., H. Lazarus, S. Farber, and G. E. Foley. 1966. Sensitivty of cultured human lymphoblasts (CCRF-CEM cells) to inhibition by thymidine. Expt. Cell Res. 43: 512–514.

    Article  CAS  Google Scholar 

  24. Vaughan, M. H., and M. W. Steele. 1971. Differential sensitivity of human normal and malignant cells to 8-azahypoxanthine in vitro. Exp. Cell Res. 69: 92–96.

    Article  PubMed  CAS  Google Scholar 

  25. Krishan, A., and R. Ray-Chaudhuri. 1970. Chromosome studies of cell lines and tumors derived from a single specimen of human leukemic blood by cell culture and heterotransplantation. Cancer Res. 30: 2012–2016.

    PubMed  CAS  Google Scholar 

  26. Adams, R. A., G. E. Foley, B. G. Uzman, S. Farber, H. Lazarus, and L. Kleinman. 1967. Leukemia: Serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772–783.

    PubMed  CAS  Google Scholar 

  27. Adams, R. A., G. E. Foley, H. Lazarus, S. Oppenheim, and A. Babcock. 1970. Antilymphocyte serum stimulation of immunoglobulin biosynthesis in cultured Burkitt's tumor (EB3) cells. Fed. Proc. 29: 301.

    Google Scholar 

  28. Lazarus, H., S. Oppenheim, and G. E. Foley. To be published.

  29. Lazarus, H., S. Oppenheim, E. F. Barell, and G. E. Foley. To be published.

  30. Tanigaki, N., Y. Yagi, G. E. Moore, and D. Pressman. 1966. Immunoglobulin production in human leukemia cell lines. J. Immunol. 97: 634–646.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Children's Cancer Research Foundation, Harvard Medical School, 02115, Boston, Massachusets

    Herbert Lazarus, E. F. Barell, S. Oppenheim & Awtar Krishan

  2. Department of Pathology, Harvard Medical School, 02115, Boston, Massacusetts

    Herbert Lazarus, E. F. Barell, S. Oppenheim & Awtar Krishan

Authors
  1. Herbert Lazarus
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. F. Barell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Oppenheim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Awtar Krishan
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

These studies were suported in part by research Grants C-6516 from the National Cancer Institute and FR-05526 from the Division of Research Facilities and Resources, National Institutes of Health.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lazarus, H., Barell, E.F., Oppenheim, S. et al. Divergen properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood. In Vitro 9, 303–310 (1974). https://doi.org/10.1007/BF02616012

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02616012

Keywords

Search

Navigation