Summary
The transport of human γ-globulin labelled with I125, and ferritin, across the rabbit yolk sac splanchnopleur, has been followed using electronmicroscopy combined with autoradiography. Both ferritin, and human γ-globulin I125 as indicated by grains, became similarly localised in the same absorptive vesicles present in the yolk sac endoderm. Only human γ-globulin I125 could be convincingly demonstrated in the basement membrane of the yolk sac endoderm, in the vascular mesenchyme, and in the vitelline vessels. These findings are discussed in the light of other studies using fluorescent protein tracing to locate simultaneously transmitted and non-transmitted proteins, and in the light of suggested mechanisms, to explain selective transmission.
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Supported by an award from the Science Research Council, to whom grateful acknowledgement is made.
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Wild, A.E., Stauber, V.V. & Slade, B.S. Simultaneous localisation of human γ-globulin I125 and ferritin during transport across the rabbit yolk sac splanchnopleur. Z.Zellforsch 123, 168–177 (1971). https://doi.org/10.1007/BF02583471
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DOI: https://doi.org/10.1007/BF02583471