Summary
Serum bone Gla-protein (BGP or osteocalcin) was measured in 25 women with histologically confirmed postmenopausal osteoporosis before and during long-term treatment with 1 μg/day of 1,25-dihydroxyvitamin D3(1,25(OH)2D3).
Basal serum BGP was significantly lower in osteoporotic women (3.8±1.4 ng/ml) than in agematched controls (6.8±2.0 ng/ml). During 1,25(OH)2D3 therapy serum BGP increased so that the mean of the values observed on treatment (4.8±1.5) was significantly higher than the mean basal value.
It is known that BGP synthesis is stimulated by 1,25 (OH)2D3 and that serum BGP is a specific marker of bone formation; therefore, it is possible that the low basal levels of osteocalcin we observed were related to the low serum 1,25(OH)2D concentrations reported in osteoporotic women and that the increase in BGP levels observed under 1,25(OH)2D3 treatment was a consequence of osteoblast stimulation.
Similar content being viewed by others
References
Hauschka PV, Lian JB, Gallop PM (1975) Direct identification of the calcium binding amino acid γ-carboxyglutamate in mineralized tissue. Proc Natl Acad Sci USA 72:3925–3929
Price PA, Otsuka AS, Poser J, Kristaponis J, Raman N (1976) Characterization of γ-carboxyglutamic acid-containing protein from bone. Proc Natl Acad Sci USA 73:1447–1451
Poser JW, Price PA (1979) A method for decarboxylation of γ-carboxyglutamic acid in protein. J Biol Chem 254:431–436
Price PA, Williamson MK, Lothringer JW (1981) Origin of the vitamin K-dependent bone protein found in plasma and its clearance by kidney and bone. J Biol Chem 256:12760–12766
Price PA, Bankol SA (1980) 1,25-dihydroxyvitamin D3 increases synthesis of the vitamin K-dependent bone protein by osteosarcoma cells. J Biol Chem 255:11660–11663
Price PA, Parthemore JG, Deftos LJ (1980) A new biochemical marker for bone metabolism. J Clin Invest 66:878–883
Gundberg CM, Lian JB, Galloy PM, Steinberg JJ (1983) Urinary γ-carboxy glutamic acid and serum osteocalcin as bone markers: studies in osteoporosis and Paget's disease. J Clin Endocrinol Metab 57:1221–1225
Delmas PD, Wahner HW, Mann KG, Riggs BL (1983) Assessment of bone turnover in postmenopausal osteoporosis by measurement of serum bone Gla-protein. J Lab Clin Med 102:470–476
Epstein S, Poser J, McClintock R, Johnston CC, Bryce G, Hui S (1984) Differences in serum bone Gla-protein with age and sex. Lancet i:307–310
Brown JP, Delmas PD, Malaval L, Edouard C, Chapuy MC, Meunier PJ (1984) Serum bone Gla-protein: a specific marker for bone formation in postmenopausal osteoporosis. Lancet i:1091–1093
Fiore CE, Malatino LS, Tamburino G (1984) La bone Gla-protein (osteocalcina): un nuovo marker metabolico dei tessuti calcificati. Rec Progr Med 75:361–378
Galli M, Caniggia M (1984) La osteocalcina. Minerva Med 75:2489–2501
Delmas PD, Stenner S, Wahner HW, Mann KG, Riggs BL (1983) Increase in serum bone-carboxyglutamic acid protein with aging in women. J Clin Endocrinol Metab 71:1316–1321
Deftos LJ, Parthemore JG, Price PA (1982) Changes in plasma bone Gla protein during treatment of bone disease. Calcif Tissue Int 34:121–124
Price PA, Nishimoto SK (1980) Radioimmunoassay for the vitamin K-dependent protein of bone and its discovery in plasma. Proc Natl Acad Sci USA 77:2234–2238
Caniggia A, Vattimo A (1979) Effect of 1,25-dihydroxycholecalciferol on 47-Calcium absorption in post-menopausal osteoporosis. Clin Endocrinol 11:99–109
Caniggia A, Nuti R, Loré F, Vattimo A (1984) The hormonal form of vitamin D in the pathophysiology and therapy of postmenopausal osteoporosis. J Endocrinol Invest 7:373–378
Caniggia A, Nuti R, Loré F, Vattimo A (1984) 1,25(OH)2 vitamin D3 in the long-term treatment of postmenopausal osteoporosis. In: Martini L, Gordon GS, Sciarra F (eds) Steroid modulation of neuroendocrine function. Sterols, steroids and bone metabolism. Elsevier Science Publishers, Amsterdam, pp 231–244
Caniggia A, Gennari C, Bianchi V, Guideri R (1963) Intestinal absorption of 45-Ca in senile osteoporosis. Acta Med Scand 173:613–617
Gallagher JC, Riggs BL, Eisman J, Hamstra A, Arnaud SB, De Luca HF (1979) Intestinal calcium absorption and serum vitamin D metabolites in normal subjects and osteoporotic patients: effect of age and dietary calcium. J Clin Invest 64:729–736
Loré F, Nuti R, Vattimo A, Caniggia A (1984) Vitamin D metabolites in postmenopausal osteoporosis. Horm Metabol Res 16:58
Jensen GF, Christiansen C, Transbol I (1982) Treatment of post-menopausal osteoporosis. A controlled therapeutic trial comparing ostrogen-gestagen, 1,25-dihydroxy-vitamin D3 and calcium. Clin Endocrinol (Oxf.) 16:515–524
Gallagher JC, Recker R (1985) A comparison between high dose calcitriol and calcium supplements in osteoporosis. In Norman AW, Schaefer K, Grigoleit HG (eds) Vitamin D, Chemical, Biochemical and Clinical Update. De Gruyter, Berlin pp 971–975
Riggs BL (1985) Role of vitamin D-endocrine system in the pathogenesis of involutional osteoporosis. In Norman AW, Schaefer K, Grigoleit HG (eds) Vitamin, D, Chemical, Biochemical and Clinical Update. De Gruyter, Berlin pp 976–985
Nordin BEC, Morris HA, Fraser V, Hartley TF, Bridges A, Walker C (1985) Relation between serum 1,25 dihydroxyvitamin D, calcium absorption and bone density in normal and osteoporotic postmenopausal women. In Norman AW, Schaefer K, Grigoleit HG (eds) Vitamin D, Chemical, Biochemical and clinial Update. De Gruyter, Berlin pp 1002–1003
Gundberg CM, Cole DEC, Lian JB, Reade TM, Gallop PM (1983) Serum osteocalcin in the treatment of inherited rickets with 1,25-dihydroxyvitamin D3. J Clin Endocrinol Metab 56:1063–1067
Zerwekh JE, Sakhaee K, Pak CYC (1985) Short-term, 1,25-dihydroxyvitamin D3 administration raises serum osteocalcin in patients with postmenopausal osteoporosis. J Clin Endocrinol Metab 60:615–617
Nuti R, Galli M, Turchetti V, Righi G, Caniggia A (1985) Effect of a short-term treatment with 1,25(OH)2D3 on Gla protein in humans. In Norman AW, Schaefer K, Grigoleit HG (eds) Vitamin D, Chemical, Biochemical and Clinical Update. De Gruyter, Berlin pp 449–450
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Caniggia, A., Nuti, R., Galli, M. et al. Effect of a long-term treatment with 1,25-dihydroxyvitamin D3 on osteocalcin in postmenopausal osteoporosis. Calcif Tissue Int 38, 328–332 (1986). https://doi.org/10.1007/BF02555745
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02555745