Abstract
The changes in the activity of NADPH-d and energy metabolism enzymes, lactate dehydrogenase (LDG) and succinate dehydrogenase (SDG), in the neurons of splanchnic and myenteric plexus (SP and MP, respectively), induced by 1-h-long ischemization of a part of the small intestine, were studied using cytophotometric technique; the measurements were performed under conditions when synthesis of nitric oxide (NO) was either blocked or activated. The activity of NADPH-d, SDG, and LDG in MP neurons was shown to be enhanced by ischemia. In SP neurons, the LDG activity increased, while the NADPH-d and SDG activities did not change. The blockade of NO synthesis with nitro-L-arginine methyl ester was followed by a decrease in the NADPH-d level in SP and MP neurons, but was maintained at a level lower than the control one during ischemia. Administration of L-arginine, the NO precursor, increased NADPH-d activity in MP and SP neurons, while at ischemization of the intestine this activity remained at a level higher than in the control. It is concluded that NO-ergic mechanisms (mostly at the MP level) are significantly involved in regulation of the functions of the small intestine in ischemia.
Similar content being viewed by others
References
V. V. Soltanov,Mechanisms of Self-Regulation of Autonomic Functions Under Normal and Pathological Conditions [in Russian], Nauka i Tekhnika, Minsk (1994).
K. McConalogue and I. B. Furness, “Projections of nitric oxide synthesizing neurons in the guinea-pig,”Tissue Res.,271, No. 3, 545–553 (1993).
X. Y. Wang, W. C. Wong, and E. A. Ling, “Localization of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) activity in the gastrointestinal sphincters in the guinea-pig,”J. Auton. Nerv. Syst.,58, Nos. 1/2, 51–55 (1996).
S. R. Brave, J. F. Tucher, A. Gibson, et al., “Localization of nitric oxide synthase within non-adrenergic non-cholinergic nerves in the mouse anococcygeus,”Neurosci. Lett.,161, No. 1, 93–96 (1993).
A. L. Salzman, “Nitric oxide in the gut,”New Horiz.,3, No. 2, 352–364 (1995).
V. P. Reutov, E. G. Sorokina, and V. E. Kositsin,Cyclic Transformations of Nitric Oxide in Mammalian Organism [in Russian], Nauka, Moscow (1997).
A. Belai, H. H. Schmidt, C. H. Hoyle, et al., “Co-localization of nitric oxide synthase and NADPH diaphorase in the myenteric plexus of the rat gut,”Neurosci. Lett.,143, 60–64 (1992).
H. M. Young, J. B. Furness, et al., “Co-localization of nitric oxide synthase immunoreactivity and NADPH-diaphorase staining in neurons of the guinea-pig intestine,”Histochemistry,97, 375–378 (1992).
M. J. Saffrey, C. J. Hassall, C. H. Hoyle, et al., “Co-localization of nitric oxide synthase and NADPH-diaphorase in cultured myenteric neurons,”NeuroReport,3, No. 4, 393–396 (1992).
B. Mazet, S. Miller, L. Ermilow, et al., “Distribution of NADPH-diaphorase in abdominal prevertebral ganglia,”J. Gastrointestin. Mot.,5, No. 3, 204 (1993).
G. M. Mowe and M. D. Gershon, “Functional heterogeneity in the myenteric plexus: demonstration using cytochrome oxidase as a verified cytochemical probe of the activity of individual enteric neurons,”J. Comp. Neurol.,249, No. 3, 381–391 (1986).
S. R. Vincent and H. Kimura, “Histochemical mapping of nitric oxide synthase in the rat brain,”Neuroscience,46, No. 4, 755–784 (1992).
S. N. Olenev and L. D. Shklyaeva, “Comparative study of dehydrogenase and diaphorase activity; basis for the technique,”Arkh. Anat., Gistol., Émbriol.,75, No. 12, 59–65 (1978).
E. Pirs,Histochemistry: Theoretical and Applied [in Russian], Inostr. Lit., Moscow (1962).
T. B. Kovalskii,Quantitative Histochemistry of Dehydrogenases and Introduction into Quantitative Histochemistry of Enzymes [in Russian], Meditsina, Moscow (1978).
S. Williamson, S. Pompolo, and I. B. Furness, “GABA and nitric oxide synthase immunoreactivities are colocalized in subset of inhibitory motor neurons of the guinea-pig small intestine,”Cell Tissue Res.,284, No. 1, 29–37 (1996).
U. Keranen, S. Vanhatalo, T. Kiviluoto, et al., “Co-localization of NADPH-diaphorase reactivity and vasoactive intestinal polypeptide in human colon,”J. Auton. Nerv. Syst.,54, No. 3, 177–183 (1995).
J. Berezin, S. H. Snyder, D. S. Bredt, and E. E. Daniel, “Ultrastructural localization of nitric oxide synthase in canine small intenstine and colon,”Am. J. Physiol.,266, No. 4, Part 1, 981–989 (1994).
Z. S. Li, S. Murphy, J. B. Furness, et al., “Relationships between nitric oxide synthase, vasoactive intestinal peptide and substance P immunoreactivities in neurons of the amphibian intestine,”J. Auton. Nerv. Syst.,44, Nos. 2/3, 197–206 (1993).
R. Guo, O. Nado, S. Suita, et al., “The distribution and co-localization of nitric oxide synthase and vasoactive intestinal polypeptide in nerves of the colon with Hirschprung’s disease,”Virch. Arch.,430, No. 10, 53–61 (1997).
C. Cortesini, F. Cianchi, A. Infantino, and M. Lise, “Nitric oxide synthase and VIP distribution in enteric nervous system in idiopathic chronic constipation,”Dig. Dis. Sci.,40, No. 11, 2450–2455 (1995).
C. R. Anderson, I. B. Furness, H. L. Woodman, et al., “Characterisation of neurons with nitric oxide synthase immunoreactivity that project to prevertebral ganglia,”J. Auton. Nerv. Syst.,53, Nos. 2/3, 107–116 (1995).
P. Karila, J. Messenger, and B. Holmgren, “Nitric oxide synthase and neuropeptide-containing subpopulations of sympathetic neurons in the coeliac ganglion of the Atlantic cod, Gadus morhua, revealed by immunohistochemistry and retrograde tracing from the stomach,”J. Auton. Nerv. Syst.,66, Nos. 1/2, 35–45 (1997).
V. V. Soltanov and A. G. Chumak, “Activation of afferent fibers of the small intestine with nitric oxide during its ischemia,” in:Role of Nitric Oxide in Vital Processes [in Russian], Minsk (1998), p. 245.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lapsha, V.I., Bocharova, V.N., Utkina, L.N. et al. Intestinal ischemia-induced changes in the activity of NADPH-d and energy metabolism enzymes in rat myenteric and splanchnic plexus. Neurophysiology 31, 376–380 (1999). https://doi.org/10.1007/BF02515139
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02515139