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New LC method using radioactivity detection for analysis of toxic metabolite of acetaminophen (Paracetamol)

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Summary

An HPLC method with UV and radioactivity detection is reported for analysis of the toxic metabolite of acetaminophen (paracetamol) in human liver samples.

Acetaminophen and its oxidative metabolites were separated on an RP-18 column with aqueous NH4-acetate:methanol mobile phase. The14C-acetaminophen and its metabolites were monitored at 250 nm and by online-coupled radioactivity detection. HPLC-MS indicated the presence of the toxic metabolite—the new method proving suitable for its serial analysis.

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Authors and Affiliations

  1. Chemical Research Center of the Hungarian Academy of Sciences, P. O. Box 17, 1525, Budapest, Hungary

    E. Hazai, E. Simon-Trompler, L. Vereczkey & K. Monostory

  2. Chemical Works of Gedeon Richter, Ltd, P. O. Box 27, 1475, Budapest 10, Hungary

    G. Czira

Authors
  1. E. Hazai
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  2. E. Simon-Trompler
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  3. G. Czira
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  4. L. Vereczkey
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  5. K. Monostory
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Hazai, E., Simon-Trompler, E., Czira, G. et al. New LC method using radioactivity detection for analysis of toxic metabolite of acetaminophen (Paracetamol). Chromatographia 56, S75–S78 (2002). https://doi.org/10.1007/BF02494117

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  • DOI: https://doi.org/10.1007/BF02494117

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