Abstract
The tumor cells (0.5 ml, 1×107) of MCF-7 line were inoculated into the subcutaneous tissue or intraperitoneum of female BALB/c nude mice. Primarily tansplanted mice were treated with 17β-estradiol dipropionate (E2) in a dose of 5 mg/kg and 17α-hydroxy progesterone caproate (Pg) in a dose of 250 mg/kg once a week. After the transferable strain was established, tumors were transplanted into female and male mice treated with E2, Pg, and E2+Pg. The tumors treated with E2 or E2+Pg grew exponentially while tumors in the other group regressed. Pg was assumed to play some role in the growth of MCF-7, in the presence of estrogen. Although cytosol estrogen receptors (ERc), nuclear estrogen receptors (ERn), and progesterone receptors (PgR) were detected by dextran coatedcharcoal method and exchange assay in the growing tumors, ERn and PgR of regressing tumors was usually negative. This MCF-7 strain in nude mice may be a promising animal model for studying chemo-hormone therapy for human breast carcinomas.
This is a preview of subscription content, log in via an institution to check access.
References
Povlsen, C. O., Rygaard, J. and Jacobsen, G. K.: Chemotherapy of human malignant melanoma transplanted in the nude mice. Cancer Res. 35: 2790–2896, 1975.
Kubota, T., Shimosato, Y. and Nagai, K.: Experimental chemotherapy of carcinoma of the stomach and colon serially transplanted in nude mice. Gann. 69: 299–309, 1977.
Kubota, T., Hanatani, Y., Tsuyuki, K., Nakada, M., Asanuma, F., Okazaki, M., Ishibiki, K., Abe, O., Nakajima, E. and Maruyama, K.: Antitumor effect of 3-[(4-amino-2-methyl-5-pyrimidinyl) methyl]-1-(2-chloroethyl)-1-nitrosourea (ACNU) on human colon carcinomas transplanted into nude mice. Jpn. J. Clin. Oncol. 12: 33–42, 1982.
Shimosato, Y., Kameya, T., Nagai, K., Hirohashi, S., Koide, T., Hayashi, H. and Nomura, T.: Transplantation of human tumors in nude mice. J. Natl. Cancer Inst. 56: 1251–1260, 1976.
Hirohashi, S., Shimosato, Y., Kameya, T., Nagai, K. and Tsunematsu, R.: Hormone dependency of a serially transplantable human breast cancer (Br-10) in nude mice. Cancer Res. 37: 3184–3189, 1977.
Brünner, N. and Visfeld, J.: Histological changes following oestradiol treatment of a hormone-responsive human breast carcinoma grown in nude mice. Acta Path. Microbiol. Immunol. Scnad. Sect. A. 90: 355–362, 1982.
Soule, H. D., Varques, J., Long, A., Albert, S. and Brennan, M. J.: A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51: 1409–1416, 1973.
Horwitz, K. B. and McGuire, W. L.: Estrogen control of progesterone receptor in human breast carcinoma. J. Biol. Chem. 253: 2223–2228, 1978.
McGuire, W. L. and DeLaGarza, M.: Similarity of the estrogen receptor in human and rat mammary carcinoma. J. Clin. Endocrinol. Metab. 36: 548–552, 1973.
Shafie, S. M. and Liotta, L. A.: Formation of metastasis by human breast carcinoma cells (MCF-7) in nude mice. Cancer Letters. 11: 81–87, 1980.
Kubota, T., Hanatani, Y., Enomoto, K. and Abe, O.: Breast Carcinoma, Endocrine Therapy. pp. 44–58, Shinohara Shuppan, Tokyo, 1981. (in Japanese)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kubota, T., Kubouchi, K., Koh, Ji. et al. Human breast carcinoma (MCF-7) serially transplanted into nude mice. The Japanese Journal of Surgery 13, 381–384 (1983). https://doi.org/10.1007/BF02469523
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02469523