Abstract
Local blood flow of the bowel wall was measured by hydrogen clearance method both in the muscular and submucosal layers in the mongrel dog, separately and concurrently. A platinum wire electrode with a bare tip of 0.5 mm in length and 0.2 mm in diameter was inserted into each layer. About 10 per cent hydrogen gas was inhalated directly through the side hole attached to the endotracheal tube for 1–2 minutes and the obtained clearance curves were plotted on the semi-logarithmic scale, which was almost monoexponential.
The results obtained were as follows:
-
1)
Mean basal perfusion rate of the muscular layer of the intestine was 0.81–0.92 ml/min/gm, whereas that of the submucosal layer was 1.29–1.31 ml/min/gm, respectively. There was little difference of perfusion rates between the small and the large intestine. The results showed good correlationship with those reported by other authors.
-
2)
The effect of vasoactive substances such as vasoconstrictor and vasodilator on the local blood flow in the bowel wall was confirmed.
-
3)
This method is repeatedly applicable for measurement and also renders the information on the distribution of blood flow in the splanchnic area.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aukland, K., Bower, B.F. and Berliner, R.W.: Measurement of local blood flow with hydrogen gas,Circulation Res., 14: 164–187, 1964.
Boley, S.J., Schwartz, S., Lash, J. and Sternhill, V.: Reversible vascular occlusion of the colon,Surg. Gynec. Obstet., 116: 53–60, 1963.
Delaney, J.P.: Chronic alterations in gastrointestinal blood flow induced by vagotomy,Surg., 62: 155–157, 1967.
Hyman, E.S.: Linear system for quantitating hydrogen at a platinum electrode,Circulation Res., 9: 1093–1097, 1961.
Kety, S.S. and Schmidt, C.F.: The determination of cerebral blood flow in man by the use of nitrous oxide in low concentrations,Am. J. Physiol., 143: 53–66, 1945.
LaMorgese, J., Fein, J.M. and Schulman, K.: Polarographic and microsphere analysis of ultraregional cerebral blood flow rates in the cat. In Harper, A.M., Jennet, W.B., Miller, J.D. and Rowan, J.O.: Blood flow and metabolism in the brain. pp. 7.3–7.8, London, Churchill Livingstone, 1975.
Mackie, D.B. and Turner, M.D.: The effect of truncal vagotomy on jejunal and ileal blood flow,J. Surg. Res., 11: 356–363, 1971.
Pasztor, E., Symon, L., Dorsch, N.W. and Branston, N.M.: The hydrogen clearance method in assessment of blood flow in cortex, white matter and deep nuclei of baboons,Stroke, 4: 556–567, 1973.
Tamura, A., Asano, T., Tak, Y., Manaka, S., Hirakawa, K. and Sano, K.: Measurement of cerebral blood flow with hydrogen clearance method,Noh to Shinkie (Brain and Nerve) 30: 47–54, 1978 (in Japanese with English summary).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mishima, Y., Shigematsu, H., Horie, Y. et al. Measurement of local blood flow of the intestine by hydrogen clearance method; Experimental study. The Japanese Journal of Surgery 9, 63–70 (1979). https://doi.org/10.1007/BF02468717
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02468717