Abstract
The characteristics of cholinoreceptors located on neurons TAN1, TAN2, and TAN3 of the ground snailAchatina fulica were studied by incubation of the central ganglia in a bath with cholinotropic preparations during intracellular recording of background neuron spike activity. Acetylcholine, nicotine, the selective n-cholinoreceptor agonist suberyldicholine, and the selective m-cholinoreceptor agonist 5-methylfurmethide concentration-dependently inhibited background spike activity to the level of complete blockade at concentrations of 500 μM. The m-cholinoblocker metamizil (500 μM) completely prevented the inhibitory activity of concentrations of 5-methylfurmethide of up to 500 μM. The central n-cholinoblocker etherophen (500 μM) completely blocked the inhibitory activity of 500 μM suberyldicholine. However, metamizil and etherophen added separately only partially decreased the inhibitory effects of acetylcholine but could not completely block the effect of acetylcholine. At the same time, mixtures of metamizil and etherophen (500 μM each) completely blocked the inhibition of background spike activity induced by acetylcholine. These results show that both classes of cholinoreceptors act on TAN neurons in the same direction.
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 85, No. 4, pp. 568–574, April, 1999.
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Stepanov, I.I., Losev, N.A. Characteristics of cholinoreceptors on identified TAN neurons of the ground snailAchatina fulica . Neurosci Behav Physiol 30, 559–563 (2000). https://doi.org/10.1007/BF02462615
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DOI: https://doi.org/10.1007/BF02462615