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Flurorescent derivatives of bovine neurotensin 8–13 fragment

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Summary

Fluorescent derivatives of bovine neurotensin 8–13 fragment were prepared. For N-terminal labelling, 4-[7-hydroxycoumaryl]acetic acid (Hca), 4-[7-methoxycoumaryl]acetic acid (Mca) and 2-amino-3-[4-[7-methoxycoumaryl]]propionic acid (Amp) were used while the C-terminus of the peptide chain was elongated with Amp. The fluorescence excitation and emission spectra of the peptide derivatives were studied. Hca- and Mca/Amp-derivatives were easily distinguishable because of the 60 nm shift of their emission maxima. Compared with the natural sequence, the presence of an N-terminal label did not influence the biological potency in a longitudinal muscle strip of guinea-pig ileum, while labelling at the C-terminus considerably reduced the activity of the peptide.

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Authors and Affiliations

  1. Department of Organic Chemistry, Eötvös Loránd University, P. O. Box 32, H-1518, Budapest 112, Hungary

    Péter Kele, Eszter Illyés & Ferenc Sebestyen

  2. Research Group of Peptide Chemistry, Hungarian Academy of Sciences, P.O. Box 32, H-1518, Budapest 112, Hungary

    Gábar Mezö

  3. Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O. Box 67, H-1450, Budapest, Hungary

    Margit Dóda

  4. Gedeon Richter Pharmaceutical Works, Ltd., P.O. Box 27, H-1475, Budapest 10, Hungary

    Marianna Mák

  5. Institute of Biophysics, Semmelweis University of Medicine, P.O. Box 263, H-1444, Budapest, Hungary

    András D. Kaposi

Authors
  1. Péter Kele
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  2. Eszter Illyés
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  3. Gábar Mezö
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  4. Margit Dóda
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  5. Marianna Mák
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  6. András D. Kaposi
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  7. Ferenc Sebestyen
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Correspondence to Ferenc Sebestyen.

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Kele, P., Illyés, E., Mezö, G. et al. Flurorescent derivatives of bovine neurotensin 8–13 fragment. Lett Pept Sci 6, 235–238 (1999). https://doi.org/10.1007/BF02443510

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  • DOI: https://doi.org/10.1007/BF02443510

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